Determination methods for carbapenem-resistant Enterobacteriaceae

doi:10.3969/j.issn.1673-8640.2020.10.002

Abstract

Abstract:

The infection caused by carbapenem-resistant Enterobacteriaceae（CRE） brings a challenge for clinical treatment,and timely and accurately identifying carbapenemase plays a role in guiding clinical antibiotic application. At present,the determination methods of carbapenemase include phenotypic screening,gene determination and the methods based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）. This review focuses on various determination methods for CRE.

Key words: Carbapenem resistance, Enterobacteriaceae, Determination method

CLC Number:

R446.5

ZHOU Hongwei, HU Yanyan, ZHANG Rong. Determination methods for carbapenem-resistant Enterobacteriaceae[J]. Laboratory Medicine, 2020, 35(10): 971-973.

References 20

[1]	GUH A Y,BULENS S N,MU Y.Epidemiology of carbapenem-resistant Enterobacteriaceae in 7 US communities,2012-2013[J]. JAMA,2015,314(14):1479-1487.
[2]	汪复,朱德妹,胡付品,等. 2012年中国CHINET细菌耐药性监测[J]. 中国感染与化疗杂志,2013,13(5):321-330.
[3]	NORDMANN P,DORTET L,POIREL L.Carbapenem resistance in Enterobacteriaceae:here is the storm![J]. Trends Mol Med,2012,18(5):263-272.
[4]	DAY K M,PIKE R,WINSTANLEY T G,et al.Use of faropenem as an indicator of carbapenemase activity in the Enterobacteriaceae[J]. J Clin Microbiol,2013,51(6):1881-1886.
[5]	TEETHAISONG Y,HOBBS G,NAKOUTI I,et al.A nitrocefin disc supplemented with ertapenem for rapid screening of carbapenemase-producing Enterobacteriaceae[J]. Diagn Microbiol Infect Dis,2018,91(1):85-88.
[6]	BUSH K,PANNELL M,LOCK J L,et al.Detection systems for carbapenemase gene identification should include the SME serine carbapenemase[J]. Int J Antimicrob Agents,2013,41(1):1-4.
[7]	HUANG L,WANG X,FENG Y,et al.First identification of an IMI-1 carbapenemase-producing colistin-resistant Enterobacter cloacae in China[J]. Ann Clin Microbiol Antimicrob,2015,14:51.
[8]	Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing[S]. CLSI,2015.
[9]	VAN DER ZWALUW K,DE HAAN A,PLUISTER G N,et al. The carbapenem inactivation method(CIM),a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods[J]. PLoS One,2015,10(3):e0123690.
[10]	HUANG T D,BERHIN C,BOGAERTS P,et al.Comparative evaluation of two chromogenic tests for rapid detection of carbapenemase in Enterobacteriaceae and in Pseudomonas aeruginosa isolates[J]. J Clin Microbiol,2014,52(8):3060-3063.
[11]	BIRGY A,BIDET P,GENEL N,et al.Phenotypic screening of carbapenemases and associated β-lactamases in carbapenem-resistant Enterobacteriaceae[J]. J Clin Microbiol,2012,50(4):1295-1302.
[12]	Centers for Disease Control and Prevention. Laboratory protocol for detection of carbapenem-resistant or carbapenemase-producing,Klebsiella spp. and E. coli from rectal swabs[R]. Atlanta:CDC,2009.
[13]	SHEN Z,HU Y,SUN Q,et al.Emerging Carriage of NDM-5 and MCR-1 in Escherichia coli From Healthy People in Multiple Regions in China:A Cross Sectional Observational Study[J]. Eclinical Medicine,2018,6:11-20.
[14]	ALIZADEH N,REZAEE M A,KAFIL H S,et al.Detection of carbapenem-resistant Enterobacteriaceae by chromogenic screening media[J]. J Microbiol Methods. 2018,153:40-44.
[15]	SINGH K,MANGOLD K A,WYANT K,et al.Rectal screening for Klebsiella pneumoniae carbapenemases:comparison of real-time PCR and culture using two selective screening agar plates[J]. J Clin Microbiol,2012,50(8):2596-2600.
[16]	MONTEFERRANTE C G,SULTAN S,TEN KATE M T,et al. Evaluation of different pretreatment protocols to detect accurately clinical carbapenemase-producing Enterobacteriaceae by MALDI-TOF[J]. J Antimicrob Chemother,2016,71(10):2856-2867.
[17]	KOST K,YI J,ROGERS B,et al.Comparison of clinical methods for detecting carbapenem-resistant Enterobacteriaceae[J]. Pract Lab Med,2017,8:18-25.
[18]	SAKARIKOU C,CIOTTI M,DOLFA C,et al.Rapid detection of carbapenemase-producing Klebsiella pneumoniae strains derived from blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS)[J]. BMC Microbiol,2017,17(1):54.
[19]	NAKANO R,NAKANO A,ISHII Y,et al.Rapid detection of the Klebsiella pneumoniae carbapenemase(KPC) gene by loop-mediated isothermal amplification(LAMP)[J]. J Infect Chemother,2015,21(3):202-206.
[20]	MOORE N M,CANTÓN R,CARRETTO E,et al. Rapid identification of five classes of carbapenem resistance genes directly from Rectal swabs by use of the Xpert Carba-R assay[J]. J Clin Microbiol,2017,55(7):2268-2275.