Determination of Ureaplasma urealyticum by real-time fluorescence isothermal RNA amplification assay

doi:10.3969/j.issn.1673-8640.2017.011.023

Abstract

Abstract:

Objective To evaluate the determination of Ureaplasma urealyticum（UU） by real-time fluorescence isothermal RNA amplification assay（SAT）. Methods A total of 200 cases of urethral swabs from sperm donors were collected and determined by SAT for UU RNA,real-time fluorescence quantitation polymerase chain reaction（qRT-PCR） for UU DNA and culturing for UU pathogens. UU-positive sperm donors were treated with antibiotics,then they were determined again for UU by qRT-PCR and SAT. Results The positive rates by culturing,qRT-PCR and SAT for UU were 20.0%（40/200）,23.0%（46/200） and 22.5%（45/200）,respectively. The Kappa value was >0.75 for the consistency of culturing,qRT-PCR and SAT. After antibiotics were given for UU-positive sperm donors for 15 d,the positive rates of qRT-PCR and SAT were 39.1%（18/46） and 32.6%（15/46）,respectively. Although there was no statistical significance,the positive rate of SAT was lower than that of qRT-PCR after treatment. Conclusions Culturing,SAT and qRT-PCR can be used for UU screening. However,UU RNA determination by SAT for patients after being given antibiotics is potential.

Key words: Ureaplasma urealyticum, Real-time fluorescence isothermal RNA amplification assay, RNA, Culturing

CLC Number:

R446.5

SHI Ying, WANG Yunfeng, LU Lu, REN Jingjing, MA Dan, LI Lin. Determination of Ureaplasma urealyticum by real-time fluorescence isothermal RNA amplification assay[J]. Laboratory Medicine, 2017, 32(11): 1043-1045.

Figures/Tables 3

References 9

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方法	阳性（例）	阴性（例）	阳性率（%）	χ²值	P值
qRT-PCR	46	154	23.0	0.605	0.739
SAT	45	155	22.5	0.605	0.739
培养法	40	160	20.0

方法	阳性（例）	阴性（例）	阳性率（%）	χ²值	P值
qRT-PCR	46	154	23.0	0.605	0.739
SAT	45	155	22.5	0.605	0.739
培养法	40	160	20.0

方法	阳性（例）	阴性（例）	阳性率（%）	χ²值	P值
qRT-PCR	18	28	39.1	1.33	0.25
SAT	15	31	32.6	1.33	0.25

方法	阳性（例）	阴性（例）	阳性率（%）	χ²值	P值
qRT-PCR	18	28	39.1	1.33	0.25
SAT	15	31	32.6	1.33	0.25

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