Abstract:

Objective To evaluate the determination of Ureaplasma urealyticum（UU） by real-time fluorescence isothermal RNA amplification assay（SAT）. Methods A total of 200 cases of urethral swabs from sperm donors were collected and determined by SAT for UU RNA,real-time fluorescence quantitation polymerase chain reaction（qRT-PCR） for UU DNA and culturing for UU pathogens. UU-positive sperm donors were treated with antibiotics,then they were determined again for UU by qRT-PCR and SAT. Results The positive rates by culturing,qRT-PCR and SAT for UU were 20.0%（40/200）,23.0%（46/200） and 22.5%（45/200）,respectively. The Kappa value was >0.75 for the consistency of culturing,qRT-PCR and SAT. After antibiotics were given for UU-positive sperm donors for 15 d,the positive rates of qRT-PCR and SAT were 39.1%（18/46） and 32.6%（15/46）,respectively. Although there was no statistical significance,the positive rate of SAT was lower than that of qRT-PCR after treatment. Conclusions Culturing,SAT and qRT-PCR can be used for UU screening. However,UU RNA determination by SAT for patients after being given antibiotics is potential.

Key words: Ureaplasma urealyticum, Real-time fluorescence isothermal RNA amplification assay, RNA, Culturing