Abstract:

Objective To investigate the relationship between the expression of plasma long non-coding RNA（lncRNA）PINK1-AS and miR-532-5p and poor sleep quality and cognitive dysfunction in patients with acute ischemic stroke （AIS）. Methods A total of 129 AIS patients（AIS group）and 124 healthy subjects（healthy control group）were enrolled from June 2021 to January 2024 at Zhengzhou Central Hospital of Zhengzhou University. The clinical data were collected，and the expressions of plasma lncRNA PINK1-AS and miR-532-5p were determined. The Pittsburgh sleep quality index（PSQI）and the Montreal cognitive assessment（MoCA）were used to evaluate sleep quality and cognitive function in AIS patients for 3 months after onset，and the subjects were classified into poor and good sleep quality groups （61 and 68 cases，respectively）and cognitive dysfunction and normal cognitive function groups（40 and 89 cases，respectively）. Pearson correlation analysis was used to evaluate the correlation between various indicators. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of the relative expression levels of plasma lncRNA PINK1-AS and miR-532-5p in predicting poor sleep quality and cognitive dysfunction in AIS patients. Results The relative expression level of plasma lncRNA PINK1-AS in AIS group was higher than that in healthy control group（P<0.001），and the relative expression level of plasma miR-532-5p was lower than that in healthy control group（P<0.001）. The relative expression level of plasma lncRNA PINK1-AS in poor sleep quality group was higher than that in good sleep quality group（P<0.001），and the relative expression level of plasma miR-532-5p was lower than that in good sleep quality group（P<0.001）. There was no statistical significance in clinical data between the 2 groups（P>0.05）. The relative expression level of plasma lncRNA PINK1-AS in cognitive dysfunction group was higher than that in normal cognitive function group（P<0.001），and the relative expression level of plasma miR-532-5p was lower than that in normal cognitive function group（P<0.001），with no statistical significance in clinical data between the 2 groups（P>0.05）. The expression of plasma lncRNA PINK1-AS in AIS patients was positively correlated with PSQI score（r=0.524，P<0.05） and negatively correlated with MoCA score（r=-0.512，P<0.05）. The expression of miR-532-5p was negatively correlated with PSQI score（r=-0.528，P<0.05）and positively correlated with MoCA score（r=0.527，P<0.05）. The areas under curves （AUC） of the relative expression levels of plasma lncRNA PINK1-AS and miR-532-5p for predicting poor sleep quality in AIS patients were 0.809，0.857 and 0.928，respectively，and the AUC for predicting cognitive dysfunction in AIS patients were 0.842，0.864 and 0.940，respectively. Conclusions Plasma lncRNA PINK1-AS is highly expressed，miR-532-5p is lowly expressed in AIS patients，and they are related to poor sleep quality and cognitive dysfunction in AIS patients.

Key words: Long non-coding RNA PINK1-AS, MicroRNA-532-5p, Acute ischemic stroke, Sleep quality, Cognitive function