Abstract:

Objective To analyze contamination bacterium spectra in cord blood among pregnant women with different delivery modes and the sources of contamination when collecting,in order to improve the collection quality for cord blood.Methods A total of 11 435 specimens of cord blood from Shanghai Cord Blood Bank were collected from August 2012 to July 2014. A total of 511 isolates and their sources were identified. Results The contamination rates of anaerobic bacteria by spontaneous deliver and cesarean were 65.2%（274 cases） and 64.9%（48 cases）,and their contamination rates of aerobic bacteria were 34.8%（146 cases） and 35.1%（26 cases）. The contamination rates of anaerobic bacteria by forceps delivery were 56.3%（9 cases） and 43.8%（7 cases）. The main anaerobic bacteria were Bacteroides and Eubacterium,accounting for 69.3%. The main aerobic bacteria were Enterobacterium and Staphylococcus,accounting for 74.3%. Conclusions Anaerobic bacteria are main contamination bacteria in cord blood,and its contamination rate has no difference by different delivery modes. Colonization bacteria from genital tract are anaerobic bacteria（Bacteroides and Eubacterium） and aerobic bacteria（Enterobacterium and Staphylococcus）,which are the main contamination sources for cord blood. It should strengthen aseptic processing when collecting cord blood in order to improve its quality.

Key words: Cord blood, Contamination, Anaerobic bacteria, Aerobic bacteria