Primary investigation on the consistency of red blood cell and white blood cell counts in Shanghai, 2014

doi:10.3969/j.issn.1673-8640.2015.11.004

Abstract

Abstract: Objective

To study the consistency of red blood cell (RBC) and white blood cell (WBC) counts in Shanghai, and to provide the reference for the appropriate accuracy verification project.

Methods

A total of 2 batches of weak-fixation-treatment whole blood samples were delivered to 710 clinical laboratories in Shanghai. Every laboratory was required to finish the determination within 3 d, with 5 duplicated determination results for each batch. All the determination data were uploaded via internet for statistical analysis within scheduled time.

Results

A total of 693 clinical laboratories, with hematology analyzers (three- and five-classification instruments) spanning 12 brands and 57 models, reported their data of RBC and WBC counts. The coefficient of variation (CV) for RBC of 2 batch samples were 2.06% and 1.99%, respectively. The CV for WBC of 2 batch samples were 4.72% and 4.84%, respectively. The CV of different analyzers for RBC and WBC of 2 batch samples were 0.76%-3.18% and 1.73%-6.32%, respectively, and the means were within 95% confidence interval.

Conclusions

The counting results of RBC and WBC in the clinical laboratories in Shanghai have consistency, and fresh weak-fixation-treatment whole blood sample is acceptable for substituting fresh whole blood in the application of the accuracy verification project for RBC and WBC counts.

Key words: Red blood cell, White blood cell, Whole blood cell counting, Consistency

CLC Number:

R446.11

WANG Qing, SONG Ying, XU Lei, MIAO Yingbo, ZHU Peichao. Primary investigation on the consistency of red blood cell and white blood cell counts in Shanghai, 2014[J]. Laboratory Medicine, 2015, 30(11): 1074-1077.

Figures/Tables 5

医院级别	汇总(家)	比例(%)
三级甲等	27	3.9
三级乙等	8	1.2
三级专科	19	2.7
二级甲等	40	5.8
二级乙等	26	3.8
二级专科	70	10.1
一级	128	18.5
乡卫生院	124	17.9
民办	229	33.0
独立实验室	9	1.3
其它(疾控、血站、疗养院、食药监、旅行保健、放射门诊、大学医院、职业病防治院)	12	1.7
未定级(质子重离子医院)	1	0.1

品牌	分类类型	型号	数量(台)
Sysmex	三分类	pocH-80i	2
		pocH-100i	12
		KX-21	121
		K-4500	18
		XP-100	3
	五分类	XS-500i	86
		XS-800i	64
		XS-1000i	17
		XT-1800i	28
		XT-2000i	14
		XT-4000i	3
		XT-5000i	1
		XE-2100	64
		XN-1000B4	1
Mindray	三分类	BC-1800	9
		BC-2300	5
		BC-2800	18
		BC-2900	1
		BC-3000	60
		BC-3600CT	2
	五分类	BC-5100	4
		BC-5300	9
		BC-5310	4
		BC-5380	3
		BC-5500	2
		BC-5800	6
ABX	三分类	Micros 60
	五分类	Pentra 60	8
		Pentra 80	1
		Pentra 120	2
		PMS 60	1
		PMS CRP	1
		PXL 80	1
Backman-Coulter	三分类	ACT diff	6
		ACT diff II	7
	五分类	ACT 5diff	2
		LH-750	9
NihonKohden(光电)	三分类	Mek-6318k	21
		Mek-6400c	2
		Mek-6410	8
		Mek-6410c	1
		Mek-6410p	3
		Mek-6420p	4
	五分类	Mek-7200	1
		Mek-7222k	5
		Mek-7300	4
Abbott	三分类	Cell-Dyn 1800	5
	五分类	Cell-Dyn 3000	1
		Cell-Dyn 3700	1
Simens	五分类	Advia 120	1
		Advia 2120	3
Orphee	五分类	Mythic	1
特康	三分类	TEK-Ⅱ mini	1
英诺华	三分类	HB 7021	1
普康	三分类	PE-6100	3
优利特	三分类	URIT-2900	7
		URIT-3000	1

项目	RBC计数(×10¹²/L)		WBC计数(×10⁹/L)
项目	批号1	批号2	批号1	批号2
$x ?$	3.89	4.31	4.44	6.14
M	3.89	4.30	4.43	6.13
s	0.08	0.09	0.21	0.30
CV(%)	2.06	1.99	4.72	4.84
2.5%~97.5%范围	3.73~4.07	4.13~4.50	4.00~4.80	5.50~6.70
$x ?$ ±2s	3.73~4.05	4.13~4.49	4.02~4.86	5.54~6.74

项目	RBC计数(×10¹²/L)		WBC计数(×10⁹/L)
项目	批号1	批号2	批号1	批号2
$x ?$	3.89	4.31	4.44	6.14
M	3.89	4.30	4.43	6.13
s	0.08	0.09	0.21	0.30
CV(%)	2.06	1.99	4.72	4.84
2.5%~97.5%范围	3.73~4.07	4.13~4.50	4.00~4.80	5.50~6.70
$x ?$ ±2s	3.73~4.05	4.13~4.49	4.02~4.86	5.54~6.74

仪器组	批号1					批号2
仪器组	$x ?$	M	s	CV(%)	$x ?$ ±2s	$x ?$	M	s	CV(%)	$x ?$ ±2s
所有仪器	3.89	3.89	0.08	2.06	3.73~4.05	4.31	4.30	0.09	1.99	4.13~4.49
Sysmex三分类	3.89	3.89	0.06	1.61	3.77~4.01	4.31	4.31	0.07	1.63	4.17~4.45
Abbott	3.90	3.90	0.07	1.68	3.77~4.03	4.31	4.31	0.09	2.03	4.13~4.49
ABX三分类	3.87	3.88	0.10	2.51	3.67~4.07	4.29	4.30	0.09	2.20	4.11~4.47
Beckman-Coulter三分类	3.88	3.89	0.09	2.41	3.70~4.06	4.28	4.29	0.08	1.80	4.12~4.44
Nihonkohden三分类	3.89	3.89	0.09	2.29	3.71~4.07	4.31	4.31	0.10	2.33	4.11~4.51
Mindray三分类	3.92	3.92	0.12	3.18	3.67~4.17	4.34	4.33	0.13	3.05	4.08~4.59
ABX五分类	3.77	3.78	0.06	1.70	3.64~3.90	4.17	4.18	0.07	1.79	4.03~4.31
Simens	3.90	3.91	0.05	1.31	3.80~4.00	4.34	4.33	0.09	2.19	4.16~4.52
Beckman-Coulter五分类	3.86	3.85	0.03	0.83	3.80~3.92	4.28	4.29	0.03	0.76	4.22~4.34
Nihonkohden五分类	3.87	3.87	0.06	1.54	3.75~3.99	4.29	4.30	0.08	1.90	4.13~4.45
Sysmex XS系列	3.87	3.86	0.05	1.36	3.77~3.97	4.29	4.29	0.06	1.38	4.17~4.41
Sysmex XT系列	3.89	3.89	0.05	1.34	3.79~3.99	4.31	4.31	0.05	1.13	4.21~4.41
Sysmex XE/XN	3.94	3.94	0.04	1.12	3.85~4.03	4.36	4.36	0.05	1.07	4.26~4.46
Mindray五分类	3.86	3.85	0.07	1.79	3.72~4.00	4.27	4.26	0.07	1.73	4.13~4.41
其它	3.92	3.92	0.08	2.15	3.75~4.09	4.31	4.31	0.11	2.57	4.09~4.53

仪器组	批号1					批号2
仪器组	$x ?$	M	s	CV(%)	$x ?$ ±2s	$x ?$	M	s	CV(%)	$x ?$ ±2s
所有仪器	3.89	3.89	0.08	2.06	3.73~4.05	4.31	4.30	0.09	1.99	4.13~4.49
Sysmex三分类	3.89	3.89	0.06	1.61	3.77~4.01	4.31	4.31	0.07	1.63	4.17~4.45
Abbott	3.90	3.90	0.07	1.68	3.77~4.03	4.31	4.31	0.09	2.03	4.13~4.49
ABX三分类	3.87	3.88	0.10	2.51	3.67~4.07	4.29	4.30	0.09	2.20	4.11~4.47
Beckman-Coulter三分类	3.88	3.89	0.09	2.41	3.70~4.06	4.28	4.29	0.08	1.80	4.12~4.44
Nihonkohden三分类	3.89	3.89	0.09	2.29	3.71~4.07	4.31	4.31	0.10	2.33	4.11~4.51
Mindray三分类	3.92	3.92	0.12	3.18	3.67~4.17	4.34	4.33	0.13	3.05	4.08~4.59
ABX五分类	3.77	3.78	0.06	1.70	3.64~3.90	4.17	4.18	0.07	1.79	4.03~4.31
Simens	3.90	3.91	0.05	1.31	3.80~4.00	4.34	4.33	0.09	2.19	4.16~4.52
Beckman-Coulter五分类	3.86	3.85	0.03	0.83	3.80~3.92	4.28	4.29	0.03	0.76	4.22~4.34
Nihonkohden五分类	3.87	3.87	0.06	1.54	3.75~3.99	4.29	4.30	0.08	1.90	4.13~4.45
Sysmex XS系列	3.87	3.86	0.05	1.36	3.77~3.97	4.29	4.29	0.06	1.38	4.17~4.41
Sysmex XT系列	3.89	3.89	0.05	1.34	3.79~3.99	4.31	4.31	0.05	1.13	4.21~4.41
Sysmex XE/XN	3.94	3.94	0.04	1.12	3.85~4.03	4.36	4.36	0.05	1.07	4.26~4.46
Mindray五分类	3.86	3.85	0.07	1.79	3.72~4.00	4.27	4.26	0.07	1.73	4.13~4.41
其它	3.92	3.92	0.08	2.15	3.75~4.09	4.31	4.31	0.11	2.57	4.09~4.53

仪器组	批号1					批号2
仪器组	$x ?$	M	s	CV(%)	$x ?$ ±2s	$x ?$	M	s	CV(%)	$x ?$ ±2s
所有仪器	4.44	4.40	0.21	4.72	4.02~4.86	6.14	6.13	0.30	4.84	5.54~6.74
Sysmex三分类	4.44	4.40	0.19	4.38	4.06~4.82	6.13	6.10	0.28	4.57	5.57~6.69
Abbott	4.36	4.30	0.26	6.02	3.84~4.88	6.05	6.10	0.31	5.09	5.43~6.67
ABX三分类	4.24	4.30	0.27	6.32	3.70~4.78	5.84	5.90	0.35	5.93	5.14~6.54
Beckman-Coulter三分类	4.51	4.50	0.22	4.93	4.07~4.95	6.18	6.20	0.28	4.60	5.62~6.74
Nihonkohden三分类	4.39	4.40	0.17	3.92	4.05~4.73	6.10	6.10	0.22	3.56	5.66~6.54
Mindray三分类	4.33	4.30	0.24	5.61	3.85~4.81	5.98	6.00	0.38	6.31	5.22~6.74
ABX五分类	4.34	4.40	0.21	4.81	3.92~4.76	5.90	6.00	0.31	5.21	5.28~6.52
Simens	4.38	4.40	0.12	2.76	4.14~4.62	6.06	6.00	0.10	1.73	5.86~6.26
Beckman-Coulter五分类	4.51	4.50	0.13	2.81	4.25~4.77	6.13	6.10	0.18	2.95	5.77~6.49
Nihonkohden五分类	4.39	4.40	0.19	4.38	4.01~4.77	6.11	6.20	0.23	3.76	5.74~6.57
Sysmex XS系列	4.43	4.40	0.15	3.49	4.13~4.73	6.15	6.10	0.22	3.60	5.71~6.59
Sysmex XT系列	4.60	4.60	0.14	3.08	4.32~4.88	6.34	6.30	0.18	2.85	5.98~6.70
Sysmex XE/XN	4.65	4.70	0.15	3.24	4.35~4.95	6.41	6.40	0.22	3.39	5.97~6.85
Mindray五分类	4.47	4.50	0.13	2.90	4.21~4.73	6.16	6.20	0.15	2.44	5.86~6.46
其它	4.42	4.40	0.19	4.30	4.04~4.80	6.18	6.20	0.27	4.42	5.64~6.72

仪器组	批号1					批号2
仪器组	$x ?$	M	s	CV(%)	$x ?$ ±2s	$x ?$	M	s	CV(%)	$x ?$ ±2s
所有仪器	4.44	4.40	0.21	4.72	4.02~4.86	6.14	6.13	0.30	4.84	5.54~6.74
Sysmex三分类	4.44	4.40	0.19	4.38	4.06~4.82	6.13	6.10	0.28	4.57	5.57~6.69
Abbott	4.36	4.30	0.26	6.02	3.84~4.88	6.05	6.10	0.31	5.09	5.43~6.67
ABX三分类	4.24	4.30	0.27	6.32	3.70~4.78	5.84	5.90	0.35	5.93	5.14~6.54
Beckman-Coulter三分类	4.51	4.50	0.22	4.93	4.07~4.95	6.18	6.20	0.28	4.60	5.62~6.74
Nihonkohden三分类	4.39	4.40	0.17	3.92	4.05~4.73	6.10	6.10	0.22	3.56	5.66~6.54
Mindray三分类	4.33	4.30	0.24	5.61	3.85~4.81	5.98	6.00	0.38	6.31	5.22~6.74
ABX五分类	4.34	4.40	0.21	4.81	3.92~4.76	5.90	6.00	0.31	5.21	5.28~6.52
Simens	4.38	4.40	0.12	2.76	4.14~4.62	6.06	6.00	0.10	1.73	5.86~6.26
Beckman-Coulter五分类	4.51	4.50	0.13	2.81	4.25~4.77	6.13	6.10	0.18	2.95	5.77~6.49
Nihonkohden五分类	4.39	4.40	0.19	4.38	4.01~4.77	6.11	6.20	0.23	3.76	5.74~6.57
Sysmex XS系列	4.43	4.40	0.15	3.49	4.13~4.73	6.15	6.10	0.22	3.60	5.71~6.59
Sysmex XT系列	4.60	4.60	0.14	3.08	4.32~4.88	6.34	6.30	0.18	2.85	5.98~6.70
Sysmex XE/XN	4.65	4.70	0.15	3.24	4.35~4.95	6.41	6.40	0.22	3.39	5.97~6.85
Mindray五分类	4.47	4.50	0.13	2.90	4.21~4.73	6.16	6.20	0.15	2.44	5.86~6.46
其它	4.42	4.40	0.19	4.30	4.04~4.80	6.18	6.20	0.27	4.42	5.64~6.72

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