Multilocus sequence typing study of ESBLs-producing Escherichia coli in one hospital

doi:10.3969/j.issn.1673-8640.2015.09.017

Abstract

Abstract: Objective

To investigate the multilocus sequence typing (MLST) and genetic evolution of extended-spectrum beta-lactamase (ESBLs)-producing Escherichia coli in the Affiliated Cancer Hospital of Zhengzhou University, and to provide the reference for the monitoring of ESBLs-producing isolates' epidemic and hospital infection control.

Methods

Gene amplification and sequence analysis were performed for 7 housekeeping genes of 26 isolates of ESBLs-producing Escherichia coli, and alleles were compared with those assigned at MLST databases.

Results

A total of 16 sequence types (ST) were obtained in the 26 isolates of ESBLs-producing Escherichia coli, in which there were 3 ST69 and 3 ST131.

Conclusions

ESBLs-producing Escherichia coli has diversified genetic backgrounds, and ST131 and ST69 are the most prevalent types, while other types were scattered.

Key words: Extended-spectrum beta-lactamase, Escherichia coli, Multilocus sequence typing

CLC Number:

R378.2

PAN Jun, XU Qingxia, XIAO Weiqiang, CHANG Yanmin, SHEN Yong. Multilocus sequence typing study of ESBLs-producing Escherichia coli in one hospital[J]. Laboratory Medicine, 2015, 30(9): 939-943.

Figures/Tables 5

References 18

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管家基因	引物序列(5'-3')	长度(bp)	退火温度(℃)
adk	F:ATTCTGCTTGGCGCTCCGGG	583	54
	R:CCGTCAACTTTCGCGTATTT
fumC	F:TCACAGGTCGCCAGCGCTTC	806	54
	R:GTACGCAGCGAAAAAGATTC
gyrB	F:TCGGCGACACGGATGACGGC	911	60
	R:ATCAGGCCTTCACGCGCATC
icd	F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA	878	54
	R:GGACGCAGCAGGATCTGTT
mdh	F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	932	60
	R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT
purA	F:CGCGCTGATGAAAGAGATGA	816	54
	R:CATACGGTAAGCCACGCAGA
recA	F:CGCATTCGCTTTACCCTGACC	780	58
	R:TCGTCGAAATCTACGGACCGGA

管家基因	引物序列(5'-3')	长度(bp)	退火温度(℃)
adk	F:ATTCTGCTTGGCGCTCCGGG	583	54
	R:CCGTCAACTTTCGCGTATTT
fumC	F:TCACAGGTCGCCAGCGCTTC	806	54
	R:GTACGCAGCGAAAAAGATTC
gyrB	F:TCGGCGACACGGATGACGGC	911	60
	R:ATCAGGCCTTCACGCGCATC
icd	F:ATGGAAAGTAAAGTAGTTGTTCCGGCACA	878	54
	R:GGACGCAGCAGGATCTGTT
mdh	F:ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	932	60
	R:TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT
purA	F:CGCGCTGATGAAAGAGATGA	816	54
	R:CATACGGTAAGCCACGCAGA
recA	F:CGCATTCGCTTTACCCTGACC	780	58
	R:TCGTCGAAATCTACGGACCGGA