Analysis and significance of T lymphocyte subsets in peripheral blood of chronic hepatitis B patients with perC region 1896 mutation

doi:10.3969/j.issn.1673-8640.2013.09.020

Abstract

Abstract:

Objective To investigate the relationship of hepatitis B virus (HBV) preC region 1896 mutation with peripheral blood T lymphocyte subsets. Methods A total of 196 patients with hepatitis B were classified into 146 cases of chronic hepatitis B (CHB) group and 50 cases of hepatitis B liver cirrhosis (LC) group. CHB group was classified into 80 cases of wild-type strain CHB group (CHB Ⅰ group) and 66 cases of preC region 1896 mutation CHB group (CHB Ⅱ group). LC group was classified into 19 cases of wild-type strain LC group (LC Ⅰ group) and 31 cases of preC region 1896 mutation LC group (LC Ⅱ group). In 196 patients with hepatitis B, the specific primers of polymerase chain reaction (PCR) was used to detect the HBV preC region 1896 mutation, and the T lymphocyte subsets in peripheral blood were detected by flow cytometry. A total of 20 healthy subjects were enrolled as healthy control group. Results In 196 hepatitis B patients, the rate of HBV preC 1896 mutation was 49.5%. The mutation rate in LC group (62.0%) was higher than that in CHB group (45.2%). Compared with the healthy control group, the CD4⁺T cell absolute number of CHB group decreased significantly (P<0.01). In LC group, the absolute number and percentage of CD3⁺T cell, the absolute number of CD8⁺T cell and the absolute number and percentage of CD4⁺T cell were significantly lower than those in the healthy control group (P<0.01). Compared with CHB Ⅰ group, the CD3⁺T cell absolute number and CD4⁺T cell absolute number of CHB Ⅱ group decreased significantly (P<0.05). Compared with CHB Ⅱ group,the absolute number of CD3⁺T cell and CD4⁺T cell in LCⅡgroup had significant decrease (P<0.01). Conclusions In CHB patients with HBV preC 1896 mutation, the imbalance of T lymphocyte subsets is more serious than that of CHB patients with wild-type strain. The imbalance may participate in the pathogenesis of chronic hepatitis caused by the HBV preC region 1896 mutation.

Key words: T lymphocyte subset, perC region 1896 mutation, Hepatitis B virus, Chronic hepatitis B

CLC Number:

R446.63

MAO Huijun, QIU Jianping, LIN Feng, WANG Gang, ZHANG Min, CHEN Daihong. Analysis and significance of T lymphocyte subsets in peripheral blood of chronic hepatitis B patients with perC region 1896 mutation[J]. , 2013, 28(9): 815-819.

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