Abstract: Objective　To compare TOSOH HLC-723 G7（HLC-723 G7），Bio-Rad D-10（D-10） and Arkray ADAMSTMA1c HA-8160（HA-8160） automatic glycosylated hemoglobin（HbA1c）analyzers with same principle ion-exchange high performance liquid chromatography for the measurement of HbA1c，and evaluate the difference among the results of the 3 automatic analyzers. Methods　The 58 patients′ samples were measured for 13 d by the 3 automatic analyzers respectively，when the low-and high-level HbA1c quality control samples were qualified. The homogeneity of variance and the one-way analysis of variance were tested with the quality control samples and patients′ samples，and the results were used to investigate the differences among the 3 analyzers. The scatter plot and difference plot of the 3 analyzers were made to check the linearity and difference. The data were used for linear regression equation，and the relative difference between each 2 analyzers was evaluated. Results　There was statistical significance among the results of the 3 analyzers for low-level quality control samples（P＜0.05），and there was no statistical significance for high-level quality control samples（P＞0.05）. The relativities were good among the 3 analyzers for measuring patients′ samples（P＞0.05）. When HbA1c level fell in the range of 4.9%-11.4%，the linear equation of HA-8160 and D-10 was Y＝0.963X + 0.179，r＝0.988，r²＝0.976，and the relative difference was －0.37%- 0.72%. The linear equation of HLC-723 G7 and D-10 was Y＝0.968X+0.523，r＝0.991，r²＝0.982，and the relative difference was －0.1%- 0.8%. The linear equation of HLC-723 G7 and HA-8160 was Y＝ 0.987X + 0.480，r＝0.984，r²＝0.969，and the relative difference was －0.13%- 1.12 %. Conclusions　At usual physiological and pathological ranges，there is no significant difference among the results measured by HLC-723 G7，D-10 and HA-8160 automatic analyzers.

Key words: Glycated hemoglobin, Ion-exchange high performance liquid chromatography, Analyzers comparison