Abstract: Objective　To investigate the accuracy of different methods of antibiotic susceptibility testing for mucoid isolates of Pseudomonas aeruginosa（PA）. Methods　A total of 66 isolates of mucoid PA were detected by Kirby-Bauer（K-B） method，ATB PSE5 kits，MicroScan WalkAway 96 plus，VITEK 2 and micro-broth dilution method. The micro-broth dilution method was adopted as reference method，and the other methods were as test methods. Results　Compared to micro-broth dilution method，the complete accordances（CA） of K-B method，ATB PSE5 kits and MicroScan were 92.63%，88.89% and 89.38%. The very major errors（VME） of the 3 methods were 0.30%，0.83% and 0.34%，respectively. The major errors（ME） of the 3 methods were 3.13%，5.83% and 5.19%. The minor errors（MIE） of the 3 methods were 3.94%，4.44% and 5.07%. The results lack in VITEK 2，since the relevant data were not analyzed. Conclusions　K-B method is a reliable method for mucoid PA antibiotic susceptibility testing，especially prolonging the incubation time to 48 h or incubating in CO2 incubator for 24 h.

Key words: Mucoid Pseudomonas aeruginosa, Antibiotic susceptibility test, Micro-broth dilution method, Evaluation