Virulence characteristics of Streptococcus pneumoniae，PBP gene mutation and β-lactam resistance

doi:10.3969/j.issn.1673-8640.2025.05.006

Abstract

Abstract:

Objective To analyze the virulence characteristics of Streptococcus pneumoniae，penicillin-binding protein （PBP） gene mutation and β-lactam resistance. Methods Totally，25 clinical isolates of Streptococcus pneumoniae were collected from Tangshan Maternal and Child Health Hospital from January to December 2022. The isolates were identified by automatic microbial identification drug susceptibility instrument. The minimum inhibitory concentrations （MIC） of penicillin，amoxicillin，cefuroxime and ceftriaxone sodium were determined by microbroth dilution method. The virulence genes （Ply，LytA，NanA，PsaA and PspA）of Streptococcus pneumoniae were determined by polymerase chain reaction （PCR）. The correlation between PBP2b gene mutation and β-lactam resistance was analyzed. Results Among the 25 isolates of Streptococcus pneumoniae，21 （84.00%） isolates were isolated from sputum samples. Totally，10（40.00%）isolates were sensitive to β-lactam antibiotics. There were 15（60.00%）isolates resistant to β-lactam antibiotics，and the MIC of 3 isolates were 4-32 μg·mL^-1. The PBP2b positive rate of 25 isolates of Streptococcus pneumoniae was 100.00%. There were 5 resistant isolates with low MIC（1-2 μg·mL^-1），and serine-X-Y lysine was changed. There were 7 isolates with medium MIC（1-4 μg·mL^-1），and serine-X-Y lysine，serine-X-Y asparagine and lysine-threonine/serine-glycine were changed. There were 3 isolates with high MIC（4-32 μg·mL^-1），and there were changes of threonine 445/451-alanine/serine and lysune-threonine/serine-glycine in the conserved region of PBP2b coding sequence near SSN. The positive rates of nanA，LytA and Ply were 80.00%，and the positive rate of nanA+Ply+LytA model was the highest（56.00%）. Conclusions Threonine 445/451-alanine/serine and lysune-threonine/serine-glycine are related to β-lactam resistance in the conserved region of PBP2b coding sequence，and the virulence genes（nanA，LytA and Ply）are widely distributed.

Key words: Streptococcus pneumoniae, Penicillin-binding protein, Gene mutation, β-lactam antibiotic, Virulence gene, Drug resistance

CLC Number:

R378.1

YAO Ling, GAO Chunyan, LIU Minglei. Virulence characteristics of Streptococcus pneumoniae，PBP gene mutation and β-lactam resistance[J]. Laboratory Medicine, 2025, 40(5): 450-454.

Figures/Tables 2

References 27

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菌株编号	阿莫西林	青霉素	头孢呋辛	头孢曲松钠	判定结果
1、3、14号	0.14	0.14	0.05	0.14	敏感
9、17、19号	0.20	0.20	0.10	0.10	敏感
5、7、12、21号	0.80	0.80	0.40	0.40	敏感
11、23号	1.00	1.00	1.00	1.00	耐药
2、15号	1.00	2.00	1.00	1.00	耐药
6、13号	4.00	2.00	1.00	1.00	耐药
4号	2.00	4.00	1.00	2.00	耐药
8号	2.00	4.00	2.00	4.00	耐药
10号	2.00	2.00	1.00	2.00	耐药
16号	2.00	4.00	1.00	1.00	耐药
18、22号	2.00	4.00	2.00	4.00	耐药
20号	4.00	8.00	32.00	4.00	耐药
24、25号	16.00	8.00	8.00	4.00	耐药

菌株编号	阿莫西林	青霉素	头孢呋辛	头孢曲松钠	判定结果
1、3、14号	0.14	0.14	0.05	0.14	敏感
9、17、19号	0.20	0.20	0.10	0.10	敏感
5、7、12、21号	0.80	0.80	0.40	0.40	敏感
11、23号	1.00	1.00	1.00	1.00	耐药
2、15号	1.00	2.00	1.00	1.00	耐药
6、13号	4.00	2.00	1.00	1.00	耐药
4号	2.00	4.00	1.00	2.00	耐药
8号	2.00	4.00	2.00	4.00	耐药
10号	2.00	2.00	1.00	2.00	耐药
16号	2.00	4.00	1.00	1.00	耐药
18、22号	2.00	4.00	2.00	4.00	耐药
20号	4.00	8.00	32.00	4.00	耐药
24、25号	16.00	8.00	8.00	4.00	耐药

毒力基因组合模式	菌株数/株	百分比/%
nanA+LytA	2	8.00
nanA+Ply	1	4.00
nanA+Ply+LytA	14	56.00
nanA+Ply+LytA+PspA	2	8.00
nanA+Ply+LytA+PsaA	5	20.00
nanA+Ply+LytA+PsaA+PspA	1	4.00

毒力基因组合模式	菌株数/株	百分比/%
nanA+LytA	2	8.00
nanA+Ply	1	4.00
nanA+Ply+LytA	14	56.00
nanA+Ply+LytA+PspA	2	8.00
nanA+Ply+LytA+PsaA	5	20.00
nanA+Ply+LytA+PsaA+PspA	1	4.00