Abstract:

Objective To investigate the relationship between serum miR-370-3p and miR-152-5p expressions and clinical pathological characteristics and prognosis of ovarian cancer patients. Methods From October 2018 to October 2020，108 patients with ovarian cancer and 108 female healthy subjects in Guangyuan First People's Hospital were enrolled. The clinical data of all the subjects were collected，and the expressions of serum miR-370-3p and miR-152-5p were determined. All the patients with ovarian cancer were followed up for 36 months. According to the prognosis，they were classified into survival group （76 cases） and death group （32 cases）. Receiver operating characteristic （ROC） curve was used to evaluate the efficacy of miR-370-3p and miR-152-5p in judging the death of ovarian cancer patients. Kaplan-Meier survival curve was used to analyze the survival of patients with ovarian cancer. Cox regression analysis was used to evaluate the prognostic factors of ovarian cancer patients. Results The relative expression levels of serum miR-370-3p and miR-152-5p in ovarian cancer group were lower than those in healthy control group （P<0.001）. There was statistical significance in the International Federation of Gynecology and Obstetrics（FIGO）stage，pathological type，degree of differentiation，lymphatic metastasis and ascites between serum miR-370-3p and miR-152-5p high expression groups（≥0.75 and ≥0.76）and low expression groups （<0.75 and <0.76）（P<0.05），and there was no statistical significance for age and tumor diameter（P>0.05）. The relative expression levels of serum miR-370-3p and miR-152-5p in death group were lower than those in survival group （P<0.001）. The areas under curves（AUC）of miR-370-3p，miR-152-5p single determinations and combined determination in judging the death of ovarian cancer patients were 0.780，0.779 and 0.897，respectively. According to the optimal cut-off value obtained by ROC curve，high expression and low expression were distinguished. The survival rates of miR-370-3p and miR-152-5p high expression groups （≥0.73 and ≥0.70）were higher than those of low expression groups（<0.73 and <0.70），respectively（Log-rank χ2=13.149 and 8.554，P=0.001 and 0.003）. Multivariate Cox regression analysis showed that decreased miR-370-3p and miR-152-5p were risk factors for death in patients with ovarian cancer [ hazard ratios （HR） were 0.883 and 0.806，95% confidence intervals （CI）were 0.829-0.940 and 0.714-0.910，respectively，P<0.001]. Conclusions The expressions of serum miR-370-3p and miR-152-5p in patients with ovarian cancer are low，and they are related to the prognosis，which may be used as evaluation indicators for the prognosis of ovarian cancer patients.

Key words: MiR-370-3p, MiR-152-5p, Ovarian cancer, Clinical pathological characteristic, Prognosis