Abstract:

Pulmonary infection is a prevalent infectious disease worldwide，with high incidence and mortality rates. Early identification of pathogenic microorganism is crucial for the correct diagnosis and treatment of the disease. Traditional laboratory testing methods have problems such as long determination time，relatively narrow pathogen spectrum and low positive determination rate，which pose challenges for clinical diagnosis of pulmonary infection and rational drug use. Metagenomic next-generation sequencing （mNGS） is an emerging pathogen microorganism determination technology，featuring high efficiency，rapidity and a wide pathogen spectrum. It is particularly significant in the determination of rare or newly emerging pathogens and has gradually been applied in the auxiliary diagnosis of infectious diseases，compensating for the limitations of traditional determination methods. This review focuses on the overview of mNGS，the impact of different specimen types on determination and its application in pulmonary infection-related research，with the aim of providing a reference for clinical diagnosis of pulmonary infection.

Key words: Pulmonary infection, Metagenomic next-generation sequencing, Diagnosis, Pathogen microorganism