Roles of serum miR-129-3p and miR-144-3p in drug-resistant pulmonary tuberculosis patients

doi:10.3969/j.issn.1673-8640.2024.11.015

Abstract

Abstract:

Objective To investigate the roles of serum miR-129-3p and miR-144-3p in drug-resistant pulmonary tuberculosis patients. Methods A total of 140 drug-resistant pulmonary tuberculosis patients who underwent neoadjuvant radiotherapy and chemotherapy in Santai Hospital of North Sichuan Medical University from January 2021 to June 2022 were enrolled. According to the therapeutic effect after 6 months of treatment，they were classified into effective group （92 cases） and ineffective group （48 cases）. Totally，140 healthy subjects who matched the general information of drug-resistant pulmonary tuberculosis patients during the same period were enrolled as control group. The expression levels of serum miR-129-3p and miR-144-3p were compared. Multivariate Logistic regression analysis was used to analyze the influencing factors of therapeutic effect in drug-resistant pulmonary tuberculosis patients. Receiver operating characteristic （ROC） curve analysis was used to analyze the predictive values of serum miR-129-3p，miR-144-3p，albumin and white blood cell count on the therapeutic effect of drug-resistant pulmonary tuberculosis after 2 months of treatment. Results Compared with control group，the levels of serum miR-129-3p and miR-144-3p in drug-resistant pulmonary tuberculosis group were decreased （P<0.05）. After 2 months of treatment，the levels of serum miR-129-3p and miR-144-3p in ineffective group were lower than those in effective group （P<0.05）. Serum miR-129-3p，miR-144-3p，albumin and white blood cell count were influencing factors for the therapeutic effect of drug-resistant pulmonary tuberculosis patients （P<0.05）. After 2 months of treatment，the area under curve of the combination of serum miR-129-3p，miR-144-3p，albumin and white blood cell count in predicting the therapeutic effect of drug-resistant pulmonary tuberculosis patients was 0.956，with the sensitivity of 87.50% and the specificity of 92.39%，which was better than their single determinations （P<0.05）. Conclusions The expression levels of miR-129-3p and miR-144-3p in serum of drug-resistant pulmonary tuberculosis patients are decreased，and the combination with miR-129-3p，miR-144-3p，albumin and white blood cell count play a role in predicting the therapeutic effect of drug-resistant pulmonary tuberculosis patients.

Key words: MiR-129-3p, MiR-144-3p, Drug-resistant pulmonary tuberculosis, Therapeutic effect, Determination

CLC Number:

R446.7

LIU Jian, XU Hui, YANG Yongqiong, DENG Zhengbo. Roles of serum miR-129-3p and miR-144-3p in drug-resistant pulmonary tuberculosis patients[J]. Laboratory Medicine, 2024, 39(11): 1113-1117.

Figures/Tables 7

References 24

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基因	上游引物5'~3'	下游引物5'~3'
miR-129-3p	ACACTCCAGCTGGGAAGCCCTTACCCCAA	TGGTGTCGTGGAGTCG
miR-144-3p	ACACTCCAGCTGGGTACAGTATAGATGATGACT	CTCAACTGGTGTCGTGGA
U6	GACCTCTATGCCAACACAGT	AGTACTTGCGCTCAGGAGGA

基因	上游引物5'~3'	下游引物5'~3'
miR-129-3p	ACACTCCAGCTGGGAAGCCCTTACCCCAA	TGGTGTCGTGGAGTCG
miR-144-3p	ACACTCCAGCTGGGTACAGTATAGATGATGACT	CTCAACTGGTGTCGTGGA
U6	GACCTCTATGCCAACACAGT	AGTACTTGCGCTCAGGAGGA

组别	例数	miR-129-3p	miR-144-3p
对照组	140	1.02±0.28	1.02±0.31
耐药肺结核组	140	0.64±0.21	0.56±0.18
t值		18.846	16.174
P值		<0.001	<0.001

组别	例数	miR-129-3p	miR-144-3p
对照组	140	1.02±0.28	1.02±0.31
耐药肺结核组	140	0.64±0.21	0.56±0.18
t值		18.846	16.174
P值		<0.001	<0.001

组别	例数	年龄/岁	性别				慢性阻塞性肺疾病			糖尿病
组别	例数	年龄/岁	男/[例（%）]		女/[例（%）]		有/[例（%）]		无/[例（%）]	有/[例（%）]		无/[例（%）]
有效组	92	46.53±5.24	54（58.70）		38（41.30）		23（25.00）		69（75.00）	37（40.22）		55（59.78）
无效组	48	47.12±5.68	27（56.25）		21（43.75）		9（18.75）		39（81.25）	15（31.25）		33（68.75）
统计值		0.614	0.077				0.699			1.086
P值		0.540	0.781				0.403			0.297
组别	体重指数/（kg·m-2）			病程/年		总胆红素/（mg·L-1）		白蛋白/（g·L-1）			白细胞计数/（×109·L-1）
有效组	22.51±2.68			16.47±2.34		11.8±3.3		36.52±4.08			6.55±1.54
无效组	22.73±2.81			16.68±2.57		12.6±3.5		32.24±3.69			8.73±2.46
统计值	0.453			0.487		1.319		6.083			6.431
P值	0.651			0.627		0.189		<0.001			<0.001