Correlation of ALOX5AP gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke

doi:10.3969/j.issn.1673-8640.2017.06.007

Abstract

Abstract:

Objective To study the correlation of 5-lipoxygenase activating protein （ALOX5AP）gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke,and to provide a reference for the early diagnosis,treatment and pathogenesis of carotid plaque-related ischemic cerebral stroke. Methods A total of 737 patients with ischemic cerebral stroke were enrolled and classified into carotid plaque group and non-carotid plaque group. Multiple polymerase chain reaction （PCR） and gene sequencing were used to analyze the 8 locus polymorphisms of ALOX5AP gene. Results Triglyceride （TG）,low-density lipoprotein cholesterol （LDL-C）,non-high-density lipoprotein cholesterol （non-HDL-C）,systolic blood pressure and blood glucose（Glu） levels in carotid plaque group were higher than those in non-carotid plaque group （P<0.05）. There was no statistical significance for the genotypes and allele frequencies of 8 loci of ALOX5AP gene between carotid plaque and non-carotid plaque groups （P>0.05）. There was no statistical significance for 8 loci of ALOX5AP gene in the formation of 2 haplotypes between carotid plaque and non-carotid plaque groups （P>0.05）. Conclusions ALOX5AP gene polymorphisms have no relationship with carotid plaque formation in ischemic cerebral stroke.

Key words: 5-Lipoxygenase activating protein, Ischemic cerebral stroke, Genotype frequency, Allele frequency

CLC Number:

R446.1

WANG Wenhui, YANG Weihua, DU Yiqiao, LIANG Fadong, YANG Zhenhua. Correlation of ALOX5AP gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke[J]. Laboratory Medicine, 2017, 32(6): 474-480.

Figures/Tables 8

References 20

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基因多态性位点	上游引物	下游引物	长度（bp）
rs10507391	GGTCCCTTTGAAACCACAGA	GAATGGCATTTTGGGGTATG	260
rs4075132	ACCAGAAAGGCTCAGCTTCA	TCCGCCTGTACCCAGAATAC	280
rs4075131	TGGACAGATGCTGGTGATGT	GGAGCCCTGTGTGAAATCAT	245
rs9578196	TCTCTCCAGCTCCCTCTCAA	TTGTGGCTGTCAGGACTCAG	300
rs9315048	GCAGAAAGGAAACCCAAAGA	AATGGGAACCCAATTGAACA	241
rs9315045	CACGCCCAGCTAATTTTTGT	TGACCTTGGGACTTTCAACC	223
rs9551963	ATTTTCAACCCTGCCGTCTA	CCCTGGATCAAGGATGACAC	289
rs12429692	ATGTCCAAGCCTCTCTTTGC	TGCCCAATCACAGAAAATCA	291

基因多态性位点	上游引物	下游引物	长度（bp）
rs10507391	GGTCCCTTTGAAACCACAGA	GAATGGCATTTTGGGGTATG	260
rs4075132	ACCAGAAAGGCTCAGCTTCA	TCCGCCTGTACCCAGAATAC	280
rs4075131	TGGACAGATGCTGGTGATGT	GGAGCCCTGTGTGAAATCAT	245
rs9578196	TCTCTCCAGCTCCCTCTCAA	TTGTGGCTGTCAGGACTCAG	300
rs9315048	GCAGAAAGGAAACCCAAAGA	AATGGGAACCCAATTGAACA	241
rs9315045	CACGCCCAGCTAATTTTTGT	TGACCTTGGGACTTTCAACC	223
rs9551963	ATTTTCAACCCTGCCGTCTA	CCCTGGATCAAGGATGACAC	289
rs12429692	ATGTCCAAGCCTCTCTTTGC	TGCCCAATCACAGAAAATCA	291

位点	探针序列	长度（bp）
rs10507391_modify	P-TAGAATTGCAAAGAGAGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs10507391_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTGCAACATTGAGGTTAAT	109
rs10507391_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTGCAACATTGAGGTTAAA	111
rs4075132_modify	P-TAAGCCATTTGTTCCCTCTCTGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs4075132_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTCAAGAAAATGGTGGCCTAAG	158
rs4075132_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTCAAGAAAATGGTGGCCTAAA	160
rs4075131_modify	P-AAAAATGTTTGGGTCAGAAAGCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs4075131_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTTGTTGTGTGTAATTTAGTAT	174
rs4075131_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTTGTTGTGTGTAATTTAGTAC	176
rs9578196_modify	P-AAATCTCACCACTCACCTCAATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9578196_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATCTGTCAAAAAGAATTCG	150
rs9578196_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATCTGTCAAAAAGAATTCA	152
rs9315048_modify	P-CTAAAATTCTGATGTTCGTTCAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9315048_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCACCAGGAGGGTACAAATCCAGCCC	174
rs9315048_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCACCAGGAGGGTACAAATCCAGCCA	176
rs9315045_modify	P-TGTTTTACAGATAAGCAAAGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9315045_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAATACATACTATTATTTATTCT	178
rs9315045_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAATACATACTATTATTTATTCC	180
rs9551963_modify	P-AGCAATTCCTCCTCGGTTGGTGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9551963_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTCCGGGGGAAGGATCTCATCT	182
rs9551963_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTCCGGGGGAAGGATCTCATCG	184
rs12429692_modify	P-CCCCAAAATGCCATTCTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs12429692_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTGCCTTTCTTCTTCCTCATT	77
rs12429692_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTGCCTTTCTTCTTCCTCATA	79

位点	探针序列	长度（bp）
rs10507391_modify	P-TAGAATTGCAAAGAGAGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs10507391_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTGCAACATTGAGGTTAAT	109
rs10507391_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGTTGCAACATTGAGGTTAAA	111
rs4075132_modify	P-TAAGCCATTTGTTCCCTCTCTGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs4075132_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTCAAGAAAATGGTGGCCTAAG	158
rs4075132_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTCAAGAAAATGGTGGCCTAAA	160
rs4075131_modify	P-AAAAATGTTTGGGTCAGAAAGCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs4075131_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTTGTTGTGTGTAATTTAGTAT	174
rs4075131_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACTTTGTTGTGTGTAATTTAGTAC	176
rs9578196_modify	P-AAATCTCACCACTCACCTCAATCTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9578196_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATCTGTCAAAAAGAATTCG	150
rs9578196_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATCTGTCAAAAAGAATTCA	152
rs9315048_modify	P-CTAAAATTCTGATGTTCGTTCAAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9315048_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCACCAGGAGGGTACAAATCCAGCCC	174
rs9315048_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCACCAGGAGGGTACAAATCCAGCCA	176
rs9315045_modify	P-TGTTTTACAGATAAGCAAAGTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9315045_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAATACATACTATTATTTATTCT	178
rs9315045_G	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGAAATACATACTATTATTTATTCC	180
rs9551963_modify	P-AGCAATTCCTCCTCGGTTGGTGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs9551963_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTCCGGGGGAAGGATCTCATCT	182
rs9551963_C	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTCCGGGGGAAGGATCTCATCG	184
rs12429692_modify	P-CCCCAAAATGCCATTCTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM
rs12429692_A	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTGCCTTTCTTCTTCCTCATT	77
rs12429692_T	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCTGCCTTTCTTCTTCCTCATA	79

组别	例数	TG（mmol/L）	TC（mmol/L）	HDL-C（mmol/L）	LDL-C（mmol/L）
无斑块组	306	1.48（1.09~2.07）	4.48（3.77~5.18）	1.03（0.89~1.26）	2.80（2.14~3.44）
斑块组	431	1.63（1.20~2.39）	4.75（3.92~5.40）	1.03（0.88~1.18）	3.06（2.43~3.58）
统计值		8.626 2	5.738 0	0.838 7	7.916 4
P值		0.003 3	0.016 6	0.359 8	0.004 9
无斑块组	3.39（2.74~4.02）		18.7（16.6~20.0）	10.7（10.4~12.0）	5.16（4.60~6.11）
斑块组	3.71（2.87~4.33）		18.7（17.3~20.8）	10.7（10.0~12.0）	5.44（4.75~7.30）
统计值	9.645 6		13.471 4	0.008 2	13.892 5
P值	0.001 9		0.000 2	0.928 0	0.000 2