Abstract:

Objective To evaluate matrix-assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF MS）,Vitek 2 Compact and MicroScan WalkAway 96 plus automatic microbial identification systems in microbiological external quality assessment. Methodse A total of 61 isolates of quality control strains with different runs were identified by MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus. The results of 3 identification systems were compared. Results The accuracy rates of MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus for identifying 26 isolates of Gram-negative bacilli were 88.4%,100.0% and 84.6%,the accuracy rates for identifying 15 isolates of Gram-positive cocci were 100.0%,100.0% and 86.7%,the accuracy rates for identifying 8 isolates of yeast-like fungi were 100.0%,100.0% and 87.5%,and the accuracy rates for identifying other 12 isolates were 100.0%,83.3% and 41.7%,respectively. Conclusions The accuracy rates of the 3 identification systems for Gram-positive cocci,Gram-negative bacilli and yeast-like fungi are good. For fastidious bacteria and Gram-positive bacilli,the accuracy rates of MALDI-TOF MS and Vitek 2 Compact are higher than those of MicroScan WalkAway 96 plus.

Key words: Matrix-assisted laser desorption/ionization time of flight mass spectrometry, Vitek 2 Compact automatic microbial identification system, MicroScan WalkAway 96 plus automatic microbial identification system, Quality control, External quality assessment