Abstract:

Objective To investigate the possible pathogenesis of chronic myelogenous leukemia（CML） by analyzing the expression levels of transforming growth factor-beta 1（TGF-β1） mRNA and bcr/abl^P210 fusion gene transcription in patients with CML.Methods Real-time fluorescence quantitative polymerase chain reaction（PCR） was used to determine the expression levels of TGF-β1 mRNA and bcr/abl^P210 in peripheral blood and bone marrow of 73 patients with CML. A total of 22 healthy subjects were enrolled as healthy control group. Pearson correlation analysis was used to analyze the correlation of TGF-β1 mRNA and bcr/abl^P210. Results The expression levels of TGF-β1 mRNA and bcr/abl^P210 in peripheral blood and bone marrow of patients with CML had no statistical significance（P>0.05）. The expression levels of TGF-β1 mRNA in healthy control group were higher for 11.39 times than those in CML group（P<0.05）. The expression levels of TGF-β1 mRNA and bcr/abl^P210 had a positive correlation in CML group（r=0.53,P<0.01）. Conclusions TGF-β1 mRNA in bone marrow may be a new indicator for the diagnosis and treatment of CML.

Key words: Transforming growth factor-beta 1, bcr/ablP210, Fusion gene, Transcription