Expression of lncRNA SOX21-AS1 and miR-875-5p in glioma tissues and relationship with prognosis of patients

doi:10.3969/j.issn.1673-8640.2024.03.001

Abstract

Abstract:

Objective To investigate the expression levels of long non-coding RNA（lncRNA）SRY-box transcription factor 21 antisense RNA 1（SOX21-AS1）and miR-875-5p in glioma tissues and the relationship with the prognosis of patients. Methods Totally，77 patients with glioma in Sanmenxia Central Hospital from July 2014 to August 2018 were enrolled as glioma group，and 50 patients with brain injury were enrolled as control group. The glioma tissues and normal brain tissues that surgically removed in the 2 groups were taken，and the expression levels of lncRNA SOX21-AS1 and miR-875-5p were determined. Pearson correlation analysis was used to analyze the correlation between the expression levels of lncRNA SOX21-AS1 and miR-875-5p in glioma tissues. Kaplan-Meier curve was used to analyze the prognosis of patients with glioma. Cox regression analysis was used to analyze the factors for the prognosis of patients with glioma. Results The expression level of lncRNA SOX21-AS1 in glioma group was higher than that in control group（P<0.001），and the expression level of miR-875-5p was lower（P<0.001）. The expression levels of lncRNA SOX21-AS1 and miR-875-5p in glioma tissue had statistical significance with different World Health Organization（WHO）classifications（P<0.05）. The expression level of lncRNA SOX21-AS1 in glioma tissue was negatively correlated with miR-875-5p（r=-0.554，P<0.001）. The cumulative survival rate of patients with glioma in lncRNA SOX21-AS1 low expression group and miR-875-5p high expression group was higher than that in lncRNA SOX21-AS1 high expression group and miR-875-5p low expression group（P<0.05）. WHO classification and lncRNA SOX21-AS1 were independent risk factors that affected the poor prognosis of patients with glioma [hazard ratios（HR）were 2.266 and 2.390，95% confidence intervals（CI）were 1.452-3.536 and 1.496-3.818，P<0.001]. Conclusions The expressions of lncRNA SOX21-AS1 and miR-875-5p are related to the prognosis of patients with glioma，and both can be used as effective indicators to assess the prognosis of patients with glioma.

Key words: Long non-coding RNA SRY-box transcription factor 21 antisense RNA 1, MiR-875-5p, Glioma, Prognosis

CLC Number:

R446.1

FANG Dandong, CHENG Gang, HUANG Wei, LIU Xiaonan, MAO Jian, HOU Baosen, LIU Shimin. Expression of lncRNA SOX21-AS1 and miR-875-5p in glioma tissues and relationship with prognosis of patients[J]. Laboratory Medicine, 2024, 39(3): 209-214.

Figures/Tables 9

References 14

[1]	康鹏, 张传宝, 原林皓, 等. 钙腔蛋白基因在脑胶质瘤中的表达及其临床意义[J]. 中华神经外科杂志, 2020, 36(2):130-135.
[2]	周开甲, 刘彦伟, 赵征, 等. 骨形成蛋白2在脑胶质瘤中的表达及其在预后评估中的应用价值分析[J]. 中华神经医学杂志, 2020, 19(7):663-670.
[3]	程飞飞, 刘江, 周红军. lncRNA RGMB-AS1在脑胶质瘤预后评估中的价值[J]. 中国临床神经外科杂志, 2020, 25(4):199-202.
[4]	全凯, 周荣福, 童建国, 等. miRNA-324-5p和转录因子叉头框C1在脑胶质瘤中的表达及其临床意义[J]. 肿瘤研究与临床, 2021, 33(4):270-275.
[5]	GAI S Y, YUAN Z H. Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells[J]. Neoplasma, 2020, 67(2):333-343. DOI PMID
[6]	CHEN T, SUN L, YAO B, et al. MicroRNA-875-5p inhibits tumor growth and metastasis of hepatocellular carcinoma by targeting eukaryotic translation initiation factor 3 subunit a[J]. Oncol Rep, 2020, 44(5):2067-2079.
[7]	王宁, 于海, 宋千, 等. 胶质瘤组织lncRNA HOTAIRM1、miR-129-5p表达变化及其临床意义[J]. 山东医药, 2021, 61(10):70-73.
[8]	CHEN S, SHEN X. Long noncoding RNAs:functions and mechanisms in colon cancer[J]. Mol Cancer, 2020, 19(1):167. DOI
[9]	MIAO W, LI N, GU B, et al. LncRNA DLGAP1-AS2 modulates glioma development by up-regulating YAP1 expression[J]. J Biochem, 2020, 167(4):411-418. DOI PMID
[10]	CHEN H, CHEN J. LncRNA SOX21-AS1 promotes the growth and invasiveness of osteosarcoma cells through miR-7-5p/IRS2 regulatory network[J]. Arch Med Res, 2021, 52(3):294-303. DOI PMID
[11]	KIPKEEVA F M, MUZAFFAROVA T A, NIKULIN M P, et al. A group of miRNA as candidates for prognostic biomarkers of gastric cancer metastasis[J]. Bull Exp Biol Med, 2020, 169(1):77-80. DOI
[12]	ZHANG H, MA H, ZHANG W, et al. Increased expression of Sema3C indicates a poor prognosis and is regulated by miR-142-5p in glioma[J]. Biol Pharm Bull, 2020, 43(4):639-648. DOI PMID
[13]	KANG N, OU Y, WANG G, et al. MiR-875-5p exerts tumor-promoting function via down-regulation of CAPZA1 in esophageal squamous cell carcinoma[J]. PeerJ, 2021, 9:e10020. DOI URL
[14]	WANG J, LU Y, DING H, et al. The miR-875-5p inhibits SATB2 to promote the invasion of lung cancer cells[J]. Gene, 2018, 644(1):13-19. DOI URL

基因	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA SOX21-AS1	GGCTCAGGTTTAGGCGAGTG	AGAGGCTCTCCTACTCGTGC
GAPDH	AGCCACATCGCTCAGACAC	GCCCAATACGACCAAATCC
miR-875-5p	CACATGGACATACGGGTT	CATCTCCGATTTGTTCCCT
U6	CTTAGTTGCATGCAG	AATCGTGTATAAGTC

基因	正向引物（5'~3'）	反向引物（5'~3'）
lncRNA SOX21-AS1	GGCTCAGGTTTAGGCGAGTG	AGAGGCTCTCCTACTCGTGC
GAPDH	AGCCACATCGCTCAGACAC	GCCCAATACGACCAAATCC
miR-875-5p	CACATGGACATACGGGTT	CATCTCCGATTTGTTCCCT
U6	CTTAGTTGCATGCAG	AATCGTGTATAAGTC

组别	例数	lncRNA SOX21-AS1	miR-875-5p
对照组	50	1.06±0.35	1.03±0.34
胶质瘤组	77	2.28±0.76	0.43±0.14
t值		10.631	13.809
P值		<0.001	<0.001

组别	例数	lncRNA SOX21-AS1	miR-875-5p
对照组	50	1.06±0.35	1.03±0.34
胶质瘤组	77	2.28±0.76	0.43±0.14
t值		10.631	13.809
P值		<0.001	<0.001

自变量	单因素分析		多因素分析
自变量	HR值（95%CI）	P值	HR值（95%CI）	P值
性别	1.008（0.825~1.231）	0.938
病理类型	1.025（0.838~1.254）	0.811
肿瘤位置	0.989（0.811~1.206）	0.913
年龄	1.103（0.896~1.358）	0.355
WHO分级	2.457（1.517~3.979）	<0.001	2.266（1.452~3.536）	<0.001
肿瘤大小	1.061（0.864~1.303）	0.573
lncRNA SOX21-AS1	2.582（1.557~4.281）	<0.001	2.390（1.496~3.818）	<0.001
miR-875-5p	0.678（0.556~0.826）	<0.001	0.894（0.731~1.094）	0.277