Analysis on the molecular characteristics and drug resistance of Staphylococcus aureus isolated from Haian,Jiangsu

Abstract

Abstract:

Objective　To analyze the molecular characteristics and drug resistance of Staphylococcus aureus isolated from Haian， Jiangsu. Methods　A total of 43 isolates of Staphylococcus aureus were collected in Haian from July 2011 to July 2012， and the isolates were identified by biochemical phenotypic test and plasma coagulase test. Antimicrobial susceptibility test to 12 kinds of antibiotics was performed by disk diffusion and broth dilution methods. Panton-Valentine leukocidin（PVL） gene was detected by polymerase chain reaction（PCR）， Staphylococcal cassette chromosome mec（SCCmec） typing was conducted according to multiplex PCR， and Staphylococcus protein A（SPA） genotyping was performed by PCR and sequencing. Results　A total of 21 isolates of methicillin-resistant Staphylococcus aureus（MRSA） were detected. t030-Ⅲ was the major MRSA clone， including 2 resistant profiles， P-CX-CN-TOB-E-CC-RA and P-CX-CN-TOB-RA. Conclusions　The isolation rate of MRSA is high， and clone spread is the main reason of resistance prevalence.

Key words: Methicillin-resistant , Molecular biological characteristic, Drug resistance

CLC Number:

R378.1

DONG Deping, GU Feifei, CHEN Xu, XIAO Shuzhen, HAN Lizhong, NI Yuxing. Analysis on the molecular characteristics and drug resistance of Staphylococcus aureus isolated from Haian,Jiangsu[J]. , 2014, 29(12): 1234-1237.

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