Abstract: Objective　To evaluate dopamine and dobutamine interference in 6 biochemical assays ［creatinine(Cr)， uric acid(UA)， glucose(Glu)， total cholesterol(TC)， triglyceride(TG) and glycated serum protein(GSP)］ based on the peroxide and peroxidase reaction principle. Methods　Sera containing different concentrations of dopamine or dobutamine (100.00,50.00,25.00,12.50,6.25,3.13 and 1.57 μg/mL) were prepared. Cr,UA,Glu,TC,TG and GSP were measured on a Hitachi 7600 automatic biochemical analyzer(Hitachi 7600). Cr, UA and Glu were measured on a VITROS 5.1 FS dry type biochemical analyzer(VITROS). The interference of each concentration drug was calculated. Results　The measured 6 assays′ results decreased gradually with the increasing of the drug concentration. The negative interference of 5 assays (except GSP) measured on the Hitachi 7600 was <10% in the drug concentration ≤1.57μg/mL. The nagative interference of Cr and UA measured on the VITROS was >10% in the drug concentration ≥50 μg/mL. There was no significant interference(<10%) of Glu measured on the VITROS in all the different drug concentrations. Conclusions　Dopamine and dobutamine in the drug concentration ≥1.57 μg/mL have significant negative interference of biochemical assays (Cr, UA, Glu, TC, TG and GSP) on Hitachi 7600. The biochemical assays measured on the VITROS are less interfered by the drugs than those on the Hitachi 7600.

Key words: Dopamine, Dobutamine, Biochemical analysis, Interference