Application of fluorescent staining in fungus detection

doi:10.3969/j.issn.1673-8640.2021.012.005

Abstract

Abstract:

Objective To evaluate the application of fluorescent staining in fungus detection. Methods A total of 212 clinical samples were collected. Fluorescent staining,direct smear,Gram staining,ink staining,fungal culturing and KOH wet film were used. Results In 175 deep samples,including sputum,pharyngeal swab,leucorrhea,urine and feces,the positive rates of fungi by fluorescent staining,direct smear and Gram staining were 87.43%,78.86% and 74.29%,respectively. The positive rate of fluorescent staining was higher（P<0.05）. In 27 superficial samples of dandruff and nail dandruff,the positive rates of fungi were 85.19%,59.26% and 55.56% by fluorescent staining,KOH wet film and fungal culturing,respectively（P<0.05）. The positive rates of fungi by fluorescent staining and ink staining in 30 cerebrospinal fluid samples had no statistical significance（P>0.05）. Conclusions Fluorescent staining is simple,rapid and specific,and it has certain clinical value in the diagnosis and identification of fungi.

Key words: Fungus, Fluorescent staining, Direct smear, Gram staining, Culturing, KOH wet film, Ink staining

CLC Number:

R379

ZHAO Lin, QIAO Yun. Application of fluorescent staining in fungus detection[J]. Laboratory Medicine, 2021, 36(12): 1219-1221.

Figures/Tables 4

References 7

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样本类型	份数	荧光染色	革兰染色	直接涂片
痰	75	71（94.67）	67（89.33）	65（86.67）
咽拭子	48	45（93.75）	40（83.33）	38（79.17）
白带	30	26（86.67）	24（80.00）	22（73.33）
尿液	12	10（83.33）	8（66.67）	7（58.33）
粪便	10	9（90.00）	8（80.00）	8（80.00）
合计	175	153（87.43）	138（78.86）	130（74.29）

样本类型	份数	荧光染色	革兰染色	直接涂片
痰	75	71（94.67）	67（89.33）	65（86.67）
咽拭子	48	45（93.75）	40（83.33）	38（79.17）
白带	30	26（86.67）	24（80.00）	22（73.33）
尿液	12	10（83.33）	8（66.67）	7（58.33）
粪便	10	9（90.00）	8（80.00）	8（80.00）
合计	175	153（87.43）	138（78.86）	130（74.29）

样本类型	例数	荧光染色	KOH湿片法	真菌培养
皮屑	15	13（86.67）	9（60.00）	9（60.00）
甲屑	12	10（83.33）	7（58.33）	6（50.00）
合计	27	23（85.19）	16（59.26）	15（55.56）

样本类型	例数	荧光染色	KOH湿片法	真菌培养
皮屑	15	13（86.67）	9（60.00）	9（60.00）
甲屑	12	10（83.33）	7（58.33）	6（50.00）
合计	27	23（85.19）	16（59.26）	15（55.56）

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