Abstract:

Objective To understand the drug resistance and toxin gene carrying status of Clostridium difficile clinical isolates in Aerospace Center Hospital. Methods The stool specimens of clinical unexplained diarrhea patients were detected by TaqMan-MGB probe real-time fluorescence quantitative polymerase chain reaction（PCR） to identify genus gene and toxin gene. Anaerobic culture,isolation and drug susceptibility test were conducted for the positive specimens of tpi gene. The clinical records were analyzed retrospectively,in order to compare the difference between A⁺B⁺ isolates and A^-B⁺ isolates. Results The tpi gene of 56 specimens was positive in 335 stool specimens,the positive rate was 16.7%,in which 10（17.9%） isolates were A^-B⁺ isolates,and 46（82.1%） isolates were A⁺B⁺ isolates. The drug resistance rates to amoxicillin,metronidazole,clindamycin,vancomycin,moxifloxacin,ampicillin-sulbactam and meropenem were 7.1%,10.7%,75.0%,0,57.1%,5.4% and 8.9%,respectively. There were 29（51.8%） isolates with drug resistance to both clindamycin and moxifloxacin. There was no statistical significance in patient history,clinical drug history and laboratory data（P>0.05）. Conclusions The multiple drug resistance of Clostridium difficile in Aerospace Center Hospital is common. The clinical characteristics are not different between A⁺B⁺ isolates and A^-B⁺ isolates.

Key words: Clostridium difficile, Drug susceptibility, Toxin gene