Aptamer-based assay for biomolecules and cells

doi:10.3969/j.issn.1673-8640.2020.01.015

Abstract

Abstract:

Aptamers are DNA or RNA nucleic acid ligands that are isolated from the libraries of oligonucleotides by an in vitro selection process called systematic evolution of ligand by exponential enrichment（SELEX）. Aptasensors,which are based on the specificity of aptamer-target recognition analytical purposes,have received particular attention due to their high sensitivity and selectivity,simple instrumentation and good stability,and they have the advantages of rapid response,low cost and easy operation as well. This review summarizes the application of aptasensors for biomolecules,including small molecules,nucleic acids,bacteria and proteins. In this review,the limitations and the main problems are discussed. Furthermore,the remaining challenges and opportunities to aptasensing platforms in the determination of biomolecules are summarized.

Key words: Aptamer, Biosensor, Biomolecule, Assay

CLC Number:

R446.1

LIU Chang, LIU Fei, SHU Liqiong, QIAN Dan, TIAN Ye, PU Xiaoyun. Aptamer-based assay for biomolecules and cells[J]. Laboratory Medicine, 2020, 35(1): 70-74.

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