Abstract:

Objective To investigate the roles of microRNA143（miRNA143） and microRNA133a（miRNA133a） in the diagnosis of bladder cancer. Methods There were 50 patients with bladder cancer （bladder cancer group）,60 patients with non-bladder tumor （disease control group） and 60 healthy subjects （healthy control group）. The levels of miRNA133a and miRNA143 in urine of bladder cancer group before and after surgery and control groups were determined by polymerase chain reaction （PCR）. Receiver operating characteristic（ROC） curve was used to evaluate the roles of miRNA143 and miRNA 133a in the diagnosis of bladder cancer. Results The preoperative levels of miRNA143 and miRNA133a in bladder cancer group were lower than those in control groups（P<0.05）, and there was no statistical significance between the 2 control groups （P>0.05）. In bladder cancer group, miRNA143 and miRNA133a levels increased after surgery（P<0.05）. There was no statistical significance for miRNA143 and miRNA133a with different TNM stages（P>0.05）. ROC curve showed that the optimal cut-off value of the preoperative level of miRNA143 for the diagnosis of bladder cancer was ≤0.165,the area under curve （AUC） was 0.827,the sensitivity was 72.7%,and the specificity was 80.8%. The optimal cut-off value of the level of miRNA133a for the diagnosis of bladder cancer was ≤0.165,the AUC was 0.846,the sensitivity was 75.0%,and the specificity was 85.4%. The diagnostic performance of the combined determination of miRNA143 and miRNA133a was not improved. Conclusions There are low levels of miRNA143 and miRNA133a in patients with bladder cancer. MiRNA143 and miRNA133a are potential for the diagnosis of bladder cancer.

Key words: MicroRNA143, MicroRNA133a, Bladder cancer, Urine