Abstract:

Objective To study the role of urinary kidney injury molecule-1（KIM-1） determination in the prediction of pre-eclampsia kidney injury during mid-gestation. Methods A total of 45 pre-eclampsia patients and 45 healthy subjects were enrolled. Urine and serum samples and clinical data during mid-gestation （the 26th to 32nd gestational weeks） were collected. Urinary KIM-1 and neutrophil gelatinase-associated lipocalin（NGAL） were determined by enzyme-linked immunosorbent assay. Urinary microalbumin （mAlb） was determined by immune transmission turbidimetric assay. Creatine （Cr） was determined by Jaffe method,and serum uric acid （UA） and urea were determined by enzymic method. Logistic regression analysis was used to assess risk factors. Results No statistical significance was found in maternal age,gestational week at sampling,primipara ratio,urinary mAlb,urinary NGAL,serum Cr and serum urea between the 2 groups （P>0.05）. The body mass index（BMI） of pre-eclampsia patients before pregnancy,urinary KIM-1 and serum UA were higher than those in healthy control group（P<0.05）. After the standardization of urinary KIM-1,mAlb and NGAL according to urinary Cr,urinary KIM-1/Cr in pre-eclampsia group was higher than that in healthy control group （P<0.01）,and urinary mAlb/Cr and NGAL/Cr had no statistical significance between the 2 groups （P>0.05）. Logistic regression analysis showed that KIM-1/Cr and BMI before pregnancy were independent risk factors for pre-eclampsia [odds ratio （OR）=1.38 and 8.48,95% confidence interval （CI） 1.04-1.83 and 1.41-50.82]. Conclusions Urinary KIM-1 might be a potential biomarker in predicting pre-eclampsia kidney injury during mid-gestation.

Key words: Kidney injury molecule-1, Pre-eclampsia, Kidney injury