Key gastric cancer-related genes and signaling pathway based on NCBI Gene Expression Omnibus

doi:10.3969/j.issn.1673-8640.2018.03.013

Abstract

Abstract:

Objective To identify the key gastric cancer-related genes and signaling pathway,and to analyze the parameters in the development of gastric cancer. Methods The gastric cancer-related data from the National Center for Biotechnology Information （NCBI）Gene Expression Omnibus （GEO） were downloaded. The genes of gastric cancer tissue and control tissue were screened by GEO2R analysis platform. The Gene Ontology （GO） and the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway were performed. The influence of key genes on the survival of patients with gastric cancer was analyzed by Kaplan Meirer Plotter（KM-Plotter）. Results Based on 3 large-scale human gastric cancer datasets,1 480 differenced genes,including 879 up-regulated genes and 601 down-regulated genes, were identified. Through the analysis of GO and KEGG pathway,the differenced genes mainly enriched on cytochrome P450 family,glcuronyl transferase family and other gene clusters. Furthermore,ALDH3A1 and NEUROD1 were deemed the most important genes being related to the progression of gastric cancer. According to the expression of the important genes,there was statistical significance for survival time between high and low expression（P<0.05）. Conclusions This study demonstrates the value of using genetic data from large-scale datasets for key gastric cancer-related genes,which provides a reference for future research.

Key words: Gene Expression Omnibus, Bioinformatics analysis, Signaling pathway, Gastric cancer

CLC Number:

R446.1

ZHANG Peng, LUO Qin, WANG Tingting, YUAN Xiangliang, SHEN Lisong. Key gastric cancer-related genes and signaling pathway based on NCBI Gene Expression Omnibus[J]. Laboratory Medicine, 2018, 33(3): 242-247.

Figures/Tables 4

References 17

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分类	主要基因
细胞色素P450家族	CYP2C18、CYP2C9、CYP3A4、CYP3A5、CYP2C19
葡萄糖醛酸转移酶家族	UGT1A5、UGT1A3、UGT1A8、UGT1A9、UGT2B15
胶原蛋白家族	COL10A1、COL12A1、COL1A1、COL11A1、COL1A2、COL8A1、COL4A1
醛酮还原酶家族	AKR1、AKR1C2、AKR1C1、AKR1B10、AKR7A3
醇脱氢酶家族	ADH1A 、ADH1B、ADH7
谷胱甘肽S-转移酶家族	GSTA1、GSTA3
衰老相关上皮膜蛋白家族	CLDN1、CLDN3、CLDN4、CLDN7、CLDN18
血小板反应素	THBS1、THBS2
其他基因	MMP3、IL-8、PTGS2、PLA2G2A、SST、SSTR、NEUROD1、ALDH3A1、CA2、TFF1 、 TFF2、GKN2、BGN、KCNJ15、KCNJ16、SERPINH1、SPARC、TIMP1、PRSS1、PRSS3

分类	主要基因
细胞色素P450家族	CYP2C18、CYP2C9、CYP3A4、CYP3A5、CYP2C19
葡萄糖醛酸转移酶家族	UGT1A5、UGT1A3、UGT1A8、UGT1A9、UGT2B15
胶原蛋白家族	COL10A1、COL12A1、COL1A1、COL11A1、COL1A2、COL8A1、COL4A1
醛酮还原酶家族	AKR1、AKR1C2、AKR1C1、AKR1B10、AKR7A3
醇脱氢酶家族	ADH1A 、ADH1B、ADH7
谷胱甘肽S-转移酶家族	GSTA1、GSTA3
衰老相关上皮膜蛋白家族	CLDN1、CLDN3、CLDN4、CLDN7、CLDN18
血小板反应素	THBS1、THBS2
其他基因	MMP3、IL-8、PTGS2、PLA2G2A、SST、SSTR、NEUROD1、ALDH3A1、CA2、TFF1 、 TFF2、GKN2、BGN、KCNJ15、KCNJ16、SERPINH1、SPARC、TIMP1、PRSS1、PRSS3

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