Abstract:

Objective To analyze the transcriptome sequencing and biological information data of glioblastoma multiforme（GBM） tissues and adjacent tissues,to dig out the key genes that cause GBM and identify their functions,and to investigate the molecular regulatory mechanism of malignant proliferation,invasion and migration of GBM. Methods Totally,4 patients with GBM were enrolled,and whole-transcriptome sequencing for tumor tissues and adjacent tissues was performed. The mRNA,microRNA（miRNA）,long non-coding RNA（lncRNA） and circular RNA（circRNA） with different expressions were screened. Bio-informatics [Gene Ontology（GO） enrichment analysis,Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway analysis,transcript enrichment analysis and so on] was performed. The mRNA-miRNA-lncRNA/circRNA associated network was established,and glioma development,migration and invasion related to target molecules were found. Results There were 3 088 different mRNA between tumor tissues and adjacent tissues,of which 1 375 cases were up-regulated,and 1 713 cases were down-regulated. There were 106 different lncRNA,of which 52 cases were up-regulated,and 54 cases were down-regulated. There were 82 different circRNA,of which 32 cases were up-regulated,and 50 cases were down-regulated. There were 15 different miRNA,of which 8 cases were up-regulated,and 7 cases were down-regulated. Differentially expressed mRNA transcripts were enriched to obtain 19 significant pathways. These pathways included Shh（Sonic hedgehog） signaling pathway,glioma signaling pathway,pancreatic cancer signaling pathway,axon guidance pathway,tumor necrosis factor（TNF） signaling pathway and other related pathways. The multivariate association analysis of whole-transcriptome sequencing found that BOC was located at the key node of its regulatory pathway（has-miR-4763-5p,has-miR-6848-5p and has-miR-6860 miRNA）. The mRNA expression level of BOC in tumor tissues was higher than that in adjacent tissues. Conclusions BOC may affect the expression of downstream target genes by the regulation of related miRNA,circRNA,lncRNA and other non-coding RNA,leading to the proliferation and invasion of GBM.

Key words: Whole-transcriptome sequencing, Glioblastoma multiforme, Hedgehog signaling pathway