Abstract:

Objective To establish an inductively coupled plasma mass spectrometry（ICP-MS） for the determinations of 5 kinds of trace elements [iron（Fe）,magnesium（Mg）,calcium（Ca）,zinc（Zn） and copper（Cu）] in whole blood. Methods After whole blood samples were fully mixed and diluted to 50 times with the solution containing Triton 100 and dilute nitric acid,adding germanium（Ge） as internal standard,an ICP-MS was established for determining 5 kinds of trace elements. A total of 495 whole blood samples of healthy subjects were collected. According to sex and age（≤16 years old and >16 years old groups）,the reference intervals were established. Results The standard curve linearities were good for 5 kinds of trace elements（r=0.999 6-1.000 0）. The within-run precision was 0.31%-3.62%,and the between-run precision was 1.37%-7.15%. The recovery rate was 98.2% -112.7 %. Each element had no statistical significance between males and females（P>0.05）,but there was statistical significance between ≤16 years old and >16 years old groups（P<0.05）. The reference intervals（x±2s） in ≤16 years old group were 30.2-43.4 mg/L for Mg,47.2-69.6 mg/L for Ca,320.6-466.0 mg/L for Fe,0.64 -1.36 mg/L for Cu and 3.1-6.8 mg/L for Zn,and those in >16 years old group were 27.0-43.6 mg/L,42.6-64.6 mg/L,275. 2-530.3 mg/L,0.40-1.58 mg/L and 4.3- 8.3 mg/L,respectively. Conclusions ICP-MS can determine Fe,Mg,Ca,Zn and Cu in whole blood simultaneously,with sensitivity,simple operation,stability and accuracy.

Key words: Iron, Magnesium, Calcium, Zinc, Copper, Inductively coupled plasma mass spectrometry, Whole blood