Abstract:

Objective To evaluate the performance of GenoType MTBDRplus VER2.0 for determining Mycobacterium tuberculosis and drug resistance in sputum specimens. Methodse Smear,MGITTM 960 liquid culturing,liquid drug susceptibility test and GenoType MTBDRplus VER2.0 were used. Using MGITTM 960 liquid culturing and liquid drug susceptibility test as standards,the sensitivity,specificity of GenoType MTBDRplus VER2.0 and the consistency with MGITTM 960 liquid culturing were analyzed,and the samples with different results were further performed for sequencing. Results Compared with MGITTM 960 liquid culturing and liquid drug susceptibility test,the sensitivity and specificity of GenoType MTBDRplus VER2.0 for the determination of Mycobacterium tuberculosis were 90.85% and 94.34%. The sensitivities for sputum with smear-positive and culturing-positive and sputum with smear-negative and culturing-positive were 93.75% and 85.96%. The sensitivity and specificity of liquid drug susceptibility test to rifampin were 100.00% and 97.3%,and those to isoniazide were 84.44% and 96.94%. Conclusions GenoType MTBDRplus VER2.0 is a rapid and effective method for determining Mycobacterium tuberculosis.

Key words: GenoType MTBDRplus VER2.0, Drug suceptibility test, Mycobacterium tuberculosis