Establishment and performance evaluation of candidate reference measurement procedure for the determination of serum potassium,sodium,calcium and magnesium based on ion chromatography

doi:10.3969/j.issn.1673-8640.2017.02.017

Abstract

Abstract:

Objective To establish a reference measurement procedure for the determination of serum electrolytes（potassium,sodium,calcium and magnesium） based on ion chromatography,and to evaluate its analytical performance.Methods Samples were prepared by a simple digestion and determined by ion chromatography. Fresh sample from patients and the International Federation of Clinical Chemistry and Laboratory Medicine External Quality Assessment Scheme for Reference Laboratories in Laboratory Medicine（RELA） sample were used. The accuracy,imprecision and methodology comparison with inductively coupled plasma mass spectrometry（ICP-MS） were evaluated.Results A total of 1.0 mL of serum sample was digested with 2 mL nitric acid. The samples were redissolved and determined by ion chromatography under the optimal eluent concentration of 32 mmol/L methane-sulfonic acid. The accuracy and imprecision were <1.0%. The results were comparable with the reference values obtained by ICP-MS. Conclusions The established reference measurement procedure for the determination of serum potassium,sodium,calcium and magnesium has the advantages of simple,low-cost,high accuracy and good stability compared to ICP-MS. It may provide an effective way for traceability.

Key words: Potassium, Sodium, Calcium, Magnesium, Ion chromatography, Reference measurement procedure, Performance evaluation

CLC Number:

R446.1

ZOU Jihua, ZHANG Man, SHEN Min, TU Minmin, ZHANG Chuanbao, YAN Ying, ZOU Bingde. Establishment and performance evaluation of candidate reference measurement procedure for the determination of serum potassium,sodium,calcium and magnesium based on ion chromatography[J]. Laboratory Medicine, 2017, 32(2): 143-148.

Figures/Tables 9

References 15

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梯度	钠	钾	钙	镁
1	0.000	0.000	0.000	0.000
2	1.304	0.103	0.050	0.021
3	2.609	0.205	0.100	0.042
4	3.913	0.308	0.150	0.063
5	5.217	0.410	0.200	0.083
6	6.522	0.513	0.250	0.104

梯度	钠	钾	钙	镁
1	0.000	0.000	0.000	0.000
2	1.304	0.103	0.050	0.021
3	2.609	0.205	0.100	0.042
4	3.913	0.308	0.150	0.063
5	5.217	0.410	0.200	0.083
6	6.522	0.513	0.250	0.104

项目	线性范围（mmol/L）	回归方程	r值	检出限（μmol/L）	定量限（μmol/L）
钠	0~6.527	Y=6.268 2X+0.009 8	1.000 0	0.019	0.063
钾	0~0.518	Y=6.776 3X-0.008 0	1.000 0	0.036	0.120
镁	0~0.104	Y=12.070 5X-0.004 4	0.999 9	0.030	0.100
钙	0~0.250	Y=12.820 3X+0.004 1	1.000 0	0.036	0.121

项目	线性范围（mmol/L）	回归方程	r值	检出限（μmol/L）	定量限（μmol/L）
钠	0~6.527	Y=6.268 2X+0.009 8	1.000 0	0.019	0.063
钾	0~0.518	Y=6.776 3X-0.008 0	1.000 0	0.036	0.120
镁	0~0.104	Y=12.070 5X-0.004 4	0.999 9	0.030	0.100
钙	0~0.250	Y=12.820 3X+0.004 1	1.000 0	0.036	0.121

项目	样本	测定值（mmol/L）	CV（%）	靶值（mmol/L）	偏移（%）	IFCC等效限（%）
钠	2014年RELA-A	137.621	0.16	138.260	-0.46	± 1.25
	2014年RELA-B	150.463	0.10	151.200	-0.49
	2015年RELA-A	140.149	0.09	139.588	0.40
	2015年RELA-B	154.268	0.11	154.200	0.04
钾	2014年RELA-A	5.848	0.39	5.878	-0.51	± 2.00
	2014年RELA-B	2.943	0.37	2.938	0.16
	2015年RELA-A	5.880	0.03	5.860	0.33
	2015年RELA-B	3.148	0.88	3.133	0.50
镁	2014年RELA-A	2.014	0.39	2.032	-0.87	± 3.75
	2014年RELA-B	0.978	0.78	0.983	-0.47
	2015年RELA-A	1.927	0.32	1.928	-0.01
	2015年RELA-B	0.909	0.54	0.908	0.08
钙	2014年RELA-A	3.165	0.44	3.153	0.38	± 2.50
	2014年RELA-B	1.826	0.18	1.826	0.01
	2015年RELA-A	3.022	0.12	3.026	-0.13
	2015年RELA-B	2.000	0.48	2.001	-0.05