Abstract:

Objective To evaluate the performance of improved uricase determination procedure and its uncertainty. Method According to A Candidate Reference Method for Uric Acid in Serum： Optimization and Evaluation which was published by American Association for Clinical Chemistry （AACC）,the accuracy was improved by standard solution gravimetric method. The precision was improved by multiple centrifugation. The accuracy and precision were evaluated,and the uncertainty was evaluated according to Guide to the Expression of Uncertainty in Measurement（GUM）. Results The standard curve linear equation of uric acid was Y=0.005 74X+0.003 09（R²=0.999 99）. The standard bias [coefficient of variation （CV）] was < 1.0%. The recovery ranged from 98.5% to 101.5%. The results of the International Federation of Clinical Chemistry and Laboratory Medicine （IFCC）external quality assessment scheme for reference laboratories in laboratory medicine（RELA） samples fell in the range of target value,with bias ±1.5% and relative standard uncertainty< 2.0%. Conclusions The improved uricase determination procedure is successfully established. It may provide an effective way for routine determination traceability of serum uric acid.

Key words: Uric acid, Serum, Uricase determination procedure, Improvement, Uncertainty