Study on Th17, Treg cell and the expression of relative cytokines in psoriasis patients

doi:10.3969/j.issn.1673-8640.2015.07.011

Abstract

Abstract: Objective

To investigate the clinical significance of T helper cell 17(Th17), regulatory T (Treg) cell and the expression of relative cytokines in psoriasis patients.

Methods

Flow cytometry was used to analyze the percentages of Th17 and Treg cells in 28 patients with mild psoriasis, 25 patients with severe psoriasis and 25 healthy controls. The mRNA expressions of retinoid-related orphan receptor gamma t (RORγt) and forkhead bos p3 (Foxp3) were detected by reverse transcription polymerase chain reaction (PCR). The concentrations of interleukin 17 (IL-17) and transforming growth facfor beta (TGF-β) in plasma of psoriasis patients were detected by enzyme-linked immunosorbent assay (ELISA).

Results

The percentages of Th17,RORγt mRNA and IL-17 in psoriasis patients increased compared with healthy controls,and there was a significantly higher levels in severe group than mild group (P<0.05). The percentage of Treg cells, Foxp3 mRNA and TGF-β in severe group were significantly lower than those in healthy controls and mild group(P<0.05).

Conclusions

The percentages of Th17 and Treg cells and their relative cytokines can be used as markers of psoriasis progression.

Key words: T helper cell 17, Regulatory T cell, Psoriasis

CLC Number:

R446.63

YANG Tingting, WANG Qing, LI Zhi, PIAO Jun, PIAO Jing'ai. Study on Th17, Treg cell and the expression of relative cytokines in psoriasis patients[J]. Laboratory Medicine, 2015, 30(7): 715-719.

Figures/Tables 5

References 12

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组别	例数	RORγt	Foxp3
健康对照组	25	0.60±0.07	0.51±0.05
银屑病
轻度组	28	0.77±0.12^*	0.48±0.07
重度组	25	0.89±0.19^*#	0.43±0.08^*#

组别	例数	RORγt	Foxp3
健康对照组	25	0.60±0.07	0.51±0.05
银屑病
轻度组	28	0.77±0.12^*	0.48±0.07
重度组	25	0.89±0.19^*#	0.43±0.08^*#

组别	例数	IL-17(pg/mL)	TGF-β(pg/mL)
健康对照组	25	12.08±3.39	30.74±2.66
银屑病
轻度组	28	23.08±5.13^*	28.62±5.02
重度组	25	48.26±7.56^*#	20.60±2.61^*#

组别	例数	IL-17(pg/mL)	TGF-β(pg/mL)
健康对照组	25	12.08±3.39	30.74±2.66
银屑病
轻度组	28	23.08±5.13^*	28.62±5.02
重度组	25	48.26±7.56^*#	20.60±2.61^*#