Cervical epithelial cell samples were collected from 846 outpatients and healthy examination subjects and determined for HPV genotyping by gene chip technology and thin-prep cytology test(TCT). Biopsies under colposcopy were taken on subjects with abnormal cytology results.

The HPV positive rate was 29.55%, and the most were high-risk HPV single-infection. A total of 24 types of HPV were detected, and the most prevalence types were HPV52, 58, 16, 53, 51 and 55 in turn. The prevalences of HPV18, HPV68 and HPV59 were different between in single-infection and in multi-infection. There were 13 types of high-risk HPV, and only one low-risk HPV was detected in high-grade cervical intraepithelial neoplasia (CIN) [including cervical squamous cell carcinoma or adenocarcinomas, CIN 3 and CIN2, as ≥CIN2]. The prevalence of HPV16 ≥CIN2 was 46.88%, which was a far higher proportion than in healthy subjects and in low-grade cervical intraepithelial pathological changes (including atypical squamous cell of undetermined significance, normal and CIN1, as<CIN2) (9.63%, P<0.05).

HPV52, 58 and 16 are the most popular types in Shanghai. HPV16 is popular in ≥CIN2 group. HPV16 is the most important predictor of high-grade cervical intraepithelial pathological changes.