The significance of urinary neutrophil gelatinase-associated lipocalin in the early diagnosis of diabetic nephropathy

doi:10.3969/j.issn.1673-8640.2015.06.010

Abstract

Abstract: Objective

To investigate the significance of urinary neutrophil gelatinase-associated lipocalin (NGAL) in the diagnosis of diabetic nephropathy(DN).

Methods

A total of 120 diabetes mellitus patients were enrolled in the study. According to 24 h urine albumin excretion rate (UAER), the patients were classified into 3 groups, normal albuminuria (NA, 46 cases), microalbuminuria (MA, 39 cases) and clinical albuminuria (CA, 35 cases) groups. Another 50 healthy subjects were enrolled as normal control (NC) group. Serum NGAL concentrations were determined by enzyme-linked immunosorbent assay (ELISA). Serum creatinine (SCr) levels were determined by Jaffé reaction-rate method. Serum cystatin C (Cys C) concentrations were determined by latex-enhanced immune turbidity method. The results were analyzed comparatively.

Results

Urine NGAL concentration in NA group was significantly higher than that in NC group(P<0.05). No statistical significance was observed in concentrations of serum Cys C and SCr between NA and NC groups (P>0.05). Urinary NGAL and serum Cys C levels in MA group were significantly higher than those in NC grpup (P<0.05). No statistical significance of SCr was found between MA and NC groups (P>0.05). Urinary NGAL, serum Cys C and SCr levels in CA group were all significantly higher than those in NC group(P<0.05).

Conclusions

Urinary NGAL may be a biomarker for early renal damage in diabetes mellitus.

Key words: Neutrophil gelatinase-associated lipocalin, Cystatin C, Diabetic nephropathy

CLC Number:

R446.11

CAI Leiming, LI Qian, TAN Longyi. The significance of urinary neutrophil gelatinase-associated lipocalin in the early diagnosis of diabetic nephropathy[J]. Laboratory Medicine, 2015, 30(6): 588-590.

Figures/Tables 1

References 18

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组别	例数	NGAL (μg/mL)	Cys C (mg/L)	SCr (μmol/L)
NA组	46	1.65±0.58	0.72±0.16	75.1±20.1
MA组	39	2.12±0.99	0.97±0.39	79.7±22.9
CA组	35	3.85±1.19	1.69±0.96	131.7±28.3
NC组	50	0.59±0.31	0.68±0.17	72.7±18.7

组别	例数	NGAL (μg/mL)	Cys C (mg/L)	SCr (μmol/L)
NA组	46	1.65±0.58	0.72±0.16	75.1±20.1
MA组	39	2.12±0.99	0.97±0.39	79.7±22.9
CA组	35	3.85±1.19	1.69±0.96	131.7±28.3
NC组	50	0.59±0.31	0.68±0.17	72.7±18.7