Evaluation on the diagnostic significance of DKK1, GP73 and AFP in primary hepatic carcinoma by Logisitic regression analysis and ROC curve analysis

doi:10.3969/j.issn.1673-8640.2015.06.003

Abstract

Abstract: Objective

To investigate the diagnostic significance of serum Dickkopf-1 (DKK1), Golgi protein 73 (GP73) and alpha fetoprotein (AFP) in primary hepatic carcinoma (PHC) by Logistic regression analysis and receiver operating characteristic (ROC) curve analysis.

Methods

Enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (ECLIA) were used to determine the serum levels of DKK1,GP73 and AFP in 81 patients with PHC, 50 patients with chronic hepatitis (CH), 50 patients with liver cirrhosis (LC) and 50 healthy controls(HC). A statistical model for diagnosing PHC was established, and the results were analyzed by Logisitic regression analysis and ROC curve analysis.

Results

Serum DKK1 levels in PHC group [5.29(4.27-7.45)ng/mL]were significantly higher than those in CH group[4.11(3.82-4.52)ng/mL], LC group [3.66(3.61-4.15)ng/mL]and HC group[3.42(3.28-3.68)ng/mL](P<0.01). Serum GP73 levels in PHC group[(215.44±68.66) ng/mL]were significantly higher than those in CH group [(121.02±49.10)ng/mL], LC group[(168.39±37.91)ng/mL]and HC group[(57.36±19.94)ng/mL](P<0.05). The sensitivities and specificities of DKK1, GP73 and AFP were 71.6%, 77.8%, 58.0% and 90.0%, 86.7%, 78.7%, respectively. The model had a sensitivity of 87.7% and a specificity of 90.0%. The areas under ROC curves (AUC) of AFP, DKK1 and GP73 were 0.76, 0.86 and 0.87, respectively, and the AUC of the model was 0.93, having statistical significance with AFP, DKK1 and GP73 (P<0.01).

Conclusion

DKK1 and GP73 can be as serum markers for the diagnosis of PHC, and the combined determination of DKK1, GP73 and AFP could improve PHC diagnostic performance. Moreover, Logisitic regression analysis combined with ROC curve analysis is a simple and effective statistical method, and it can be used in the analysis and evaluation of multi-index combined diagnosis of PHC.

Key words: Dickkopf-1, Golgi protein 73, Alpha fetoprotein, Primary hepatic carcinoma, Diagnosis

CLC Number:

R446.61

ZONG Yingying, XU Hao, XU Wei, GU Yuming, ZU Maoheng, WAN Haoguang, WU Kang. Evaluation on the diagnostic significance of DKK1, GP73 and AFP in primary hepatic carcinoma by Logisitic regression analysis and ROC curve analysis[J]. Laboratory Medicine, 2015, 30(6): 559-563.

Figures/Tables 4

References 22

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组别	例数	DKK1	GP73	AFP
PHC组	81	5.29(4.277.45)	215.44±68.66	37.18(7.98427.40)
CH组	50	4.11(3.824.52)^**	121.02±49.10^*	9.76(4.4698.79)^*
LC组	50	3.66(3.614.15)^**	168.39±37.91^*	6.24(2.4679.38)^**
HC组	50	3.42(3.283.68)^**	57.36±19.94^*	6.03(0.7128.88)^**

组别	例数	DKK1	GP73	AFP
PHC组	81	5.29(4.277.45)	215.44±68.66	37.18(7.98427.40)
CH组	50	4.11(3.824.52)^**	121.02±49.10^*	9.76(4.4698.79)^*
LC组	50	3.66(3.614.15)^**	168.39±37.91^*	6.24(2.4679.38)^**
HC组	50	3.42(3.283.68)^**	57.36±19.94^*	6.03(0.7128.88)^**

变量	系数B	标准误	Wald检验	P值	OR值
AFP	0.002	0.001	5.471	0.02	1.00
DKK1	1.058	0.195	29.518	0.00	2.88
GP73	0.023	0.004	30.565	0.00	1.02
截距	-9.492	1.308	52.623	0.00	0.00

变量	系数B	标准误	Wald检验	P值	OR值
AFP	0.002	0.001	5.471	0.02	1.00
DKK1	1.058	0.195	29.518	0.00	2.88
GP73	0.023	0.004	30.565	0.00	1.02
截距	-9.492	1.308	52.623	0.00	0.00

诊断指标	AUC	标准误	95%可信区间
AFP	0.76	0.05	0.69~0.82
DKK1	0.86^*	0.04	0.82~0.91
GP73	0.87^*	0.05	0.82~0.92
Logistic 回归模型	0.93^**#△	0.03	0.89~0.97