The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients

doi:10.3969/j.issn.1673-8640.2015.04.012

Abstract

Abstract:

Objective To investigate the change and significance of serum microRNA (miR)-520g in preeclampsia among pregnant patients. Methods Serum cycling miR was extracted from 30 patients with preeclampsia and 30 healthy pregnant women at early, middle and late pregnant stages. The expression of miR-520g was determined by quantitive reverse transcription polymerase chain reaction(PCR), and the difference between the 2 groups was analyzed statistically. Results MiR-520g increased with pregnant days in healthy pregnant group. MiR-520g in early pregnant stage of preeclampsia group increased comparing with that of healthy pregnant group, whereas no significant difference was observed in middle and late pregnant stages between preeclampsia group and healthy pregnant group. Conclusions Serum miR-520g in early pregnant stage may be a potential biomarker for the prediction of preeclampsia.

Key words: Preeclampsia, MicroRNA, Serum, Biomarker

CLC Number:

Q503

LI Qian, LONG Anxiong, HONG Mao, CAI Leiming, XIE Li, GU Ji'an, TAN Longyi.. The analysis of serum miR-520g expression in the early stage of preeclampsia among pregnant patients[J]. Laboratory Medicine, 2015, 30(4): 345-348.

Figures/Tables 3

References 12

[1]	YOUNG BC, LEVINE RJ, KARUMANCHI SA.Pathogenesis of preeclampsia[J]. Annu Rev Pathol, 2010, 5:173-192.
[2]	LIANG Y, RIDZON D, WONG L, et al.Characterization of microRNA expression profiles in normal human tissues[J]. BMC Genomics, 2007, 8:166.
[3]	乐杰. 妇产科学[M].7版.北京:人民卫生出版社, 2009:92-101.
[4]	BARTEL DP.MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116(2): 281-297.
[5]	MITCHELL PS, PARKIN RK, KROH EM, et al.Circulating microRNAs as stable blood-based markers for cancer detection[J]. Proc Natl Acad Sci USA, 2008, 105(30):10513-10518.
[6]	袁小松, 张一鸣,蒋雅琴, 等. 血清可溶性血管内皮生长因子受体1/胎盘生长因子比值作为预测子痫前期患病风险的价值[J]. 检验医学, 2010, 25(10): 784-786.
[7]	PINELES BL, ROMERO R, MONTENEGRO D, et al. Distinct subsets of microRNAs are expressed differentially in the human placentas of patients with preeclampsia [J]. Am J Obstet Gynecol, 2007, 196(3):261. e1-261. e6.
[8]	ZHU XM, HAN T, SARGENT IL, et al. Differential expression profile of microRNAs in human placentas from preeclamptic pregnancies vs normal pregnancies [J]. Am J Obstet Gynecol, 2009, 200(6):661. e1-661. e7.
[9]	ENQUOBAHRIE DA, ABETEW DF, SORENSEN TK, et al. Placental microRNA expression in pregnancies complicated by preeclampsia [J]. Am J Obstet Gynecol, 2011, 204(2):178. e12-178. e21.
[10]	YANG Q, LU J, WANG S, et al.Application of next-generation sequencing technology to profile the circulating microRNAs in the serum of preeclampsia versus normal pregnant women[J]. Clin Chim Acta, 2011, 412(23-24):2167-2173.
[11]	HROMADNIKOVA I, KOTLABOVA K, DOUCHA J, et al.Absolute and relative quantification of placenta-specific micrornas in maternal circulation with placental insufficiency-related complications[J]. J Mol Diagn, 2012, 14(2): 160-167.
[12]	MOSCH B, REISSENWEBER B, NEUBER C, et al.Eph receptors and ephrin ligands:important players in angiogenesis and tumor angiogenesis[J]. J Oncol, 2010, 2010:135285.

组别	例数	年龄 (岁)	孕前BMI (kg/m²)	PE诊断孕周
PE组	30	30(23~37)	22.6±3.1	35.3±2.7
正常妊娠组	30	28(22~35)	21.3±2.6	-

组别	例数	年龄 (岁)	孕前BMI (kg/m²)	PE诊断孕周
PE组	30	30(23~37)	22.6±3.1	35.3±2.7
正常妊娠组	30	28(22~35)	21.3±2.6	-

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