Establishment of fluorescence quantitation PCR for the quantitative detection of five species of Candida

doi:10.3969/j.issn.1673-8640.2015.03.016

Abstract

Abstract: Objective

To establish and evaluate fluorescence quantitation polymerase chain reaction (PCR) for the quantitative detection of 5 species of Candida.

Methods

The presence of DNA from pathogens in the Candida species was detected by the PCR targeting internal transcribed spacer region of fungal gene. The PCR was optimized and evaluated methodologically.

Results

The fluorescence quantitation PCR showed a wide range of linearity from 10¹-10⁹ CFU/mL, high sensitivity 5 CFU/mL, within-run and between-run coefficients of variation (CV) <5%. When the PCR was applied directly to identify 19 mocked double-blind blood samples, 5 of them were positive to 5 species of Candida, and the other 14 of them were negative to other pathogens.

Conclusions

The PCR may be appropriate for clinical laboratories as accurate, rapid and reliable determination for the most frequently encountered Candida species.

Key words: Candida, Fluorescence quantitation polymerase chain reaction, Quantitation

CLC Number:

Q503

XIA Qianfeng, FU Qiongyao, WU Qiang, QIN Xi, YAO Maozhong, HUANG Mianqing, XING Guilan, QIAN Shiyun. Establishment of fluorescence quantitation PCR for the quantitative detection of five species of Candida[J]. Laboratory Medicine, 2015, 30(3): 265-268.

Figures/Tables 1

References 11

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