Abstract:

Objective To investigate the age,Candida distribution and in vitro antifungal susceptibility in patients with vulvovaginal candidiasis（VVC）in Jiading district,and to provide a reference for the diagnosis and treatment of VVC. Methods The vaginal secretions from patients with VVC in the Maternal and Children Healthcare Hospital of Jiading District from July 2015 to October 2015 were collected,and Candida were cultured and identified by CHROMagar Candida color medium and ATB microbiological identification system. All isolates were confirmed by sequencing,using internal transcribed spacer（ITS）1 and ITS4. Drug susceptibility test was performed by Sensititre Yeast One,and minimal inhibitory concentration（MIC） was determined according to the Clinical and Laboratory Standards Institute（CLSI）2012 M27-S4. Results A total of 829 isolates of Candida were isolated,and 82 isolates were collected from recurrent vulvovaginal candidiasis（RVVC） group. Adult women（20-59 years old） accounted for 97.1%. Candida albicans accounted for 77.1%（639/829）,Candida glabrata accounted for 15.1%（125/829）,Candida parapsilosis accounted for 3.4%（28/829）,Candida tropicalis accounted for 2.1%（17/829）,Candida krusei accounted for 1.6%（13/829）,and Saccharomyces cerevisiae accounted for 0.8%（7/829）. The susceptibility rates of Candida albicans in VVC group to fluconazole,itraconazole,voriconazole,flucytosine,caspofungin,anidulafungin and micafungin were 66.1%,81.4%,44.1%,96.6%,100.0%,100.0% and 100.0%,and those in RVVC group were 41.5%,53.7%,26.8%,95.1%,100.0%,100.0% and 100.0%,respectively. Conclusions Candida albicans is the main pathogenic fungus for VVC,and the drug resistance rate to azoles is high. Therefore,antifungal agents should be chosen according to the results of drug susceptibility test to improve the precision of treatment and reduce the incidence of drug resistant isolates.

Key words: Candida, Vulvovaginal candidiasis, Drug susceptibility