The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured

doi:10.3969/j.issn.1673-8640.2015.03.014

Abstract

Abstract: Objective

To investigate the clinical significance of microbial specimens being smeared and Gram stained for microscopy before they were cultured .

Methods

A total of 365 clinical specimens with different types of microorganisms were firstly smeared and stained for microscopy, then were inoculated for culture. Finally, the coincidence rate was analyzed.

Results

Among the 365 specimens, there were 211 sputum specimens, 40 urine specimens, 76 secretion specimens and 38 other-type specimens (mainly pus and pleural effusion specimens). Among them, there were 17 unqualified specimens. Of the 348 qualified specimens, 93 cases were cultured positive. The detection results of sputum, urine, secretion and other-type specimens were no statistical significance by the smear microscopy and the culture method. The coincidence rates were 76.6%, 62.5%, 87.1% and 80.0%, respectively (P>0.05).

Conclusions

The smear microscopy can not only check the quality of specimens, but also reduce the result errors of culture, so as to improve the positive rate of specimens by culture. Therefore, it is of great significance in the actual microbiological testing work.

Key words: Smear, Microscopy, Culture, Microbial specimen

CLC Number:

R446.5

ZHANG Guoying, XIA Xuehong. The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured[J]. Laboratory Medicine, 2015, 30(3): 258-261.

标本类型	菌种	例数
痰液
	肺炎克雷伯菌	19
	白念珠菌	15
	铜绿假单胞菌	4
	嗜麦芽窄食单胞菌	3
	溶血葡萄球菌	2
	其它	4
	合计	47
尿液
	屎肠球菌	3
	产气肠杆菌	1
	恶臭假单胞菌	1
	大肠埃希菌	1
	白念珠菌	1
	合计	7
分泌物
	大肠埃希菌	17
	肺炎克雷伯菌	3
	铜绿假单胞菌	2
	金黄色葡萄球菌	2
	白念珠菌	1
	肺炎链球菌	1
	其它	4
	合计	30
其它
	大肠埃希菌	4
	铜绿假单胞菌	2
	木糖葡萄球菌	1
	金黄色葡萄球菌	1
	液化沙雷菌	1
	合计	9

标本类型	菌种	例数
痰液
	肺炎克雷伯菌	19
	白念珠菌	15
	铜绿假单胞菌	4
	嗜麦芽窄食单胞菌	3
	溶血葡萄球菌	2
	其它	4
	合计	47
尿液
	屎肠球菌	3
	产气肠杆菌	1
	恶臭假单胞菌	1
	大肠埃希菌	1
	白念珠菌	1
	合计	7
分泌物
	大肠埃希菌	17
	肺炎克雷伯菌	3
	铜绿假单胞菌	2
	金黄色葡萄球菌	2
	白念珠菌	1
	肺炎链球菌	1
	其它	4
	合计	30
其它
	大肠埃希菌	4
	铜绿假单胞菌	2
	木糖葡萄球菌	1
	金黄色葡萄球菌	1
	液化沙雷菌	1
	合计	9

标本类型	涂片镜检法		培养法		符合率 (%)
标本类型	阳性	阴性	阳性	阴性	符合率 (%)
痰液	36	163	47	152	76.6
尿液	6	29	7	28	62.5
分泌物	28	48	30	46	87.1
其它	9	29	9	29	80.0

标本类型	涂片镜检法		培养法		符合率 (%)
标本类型	阳性	阴性	阳性	阴性	符合率 (%)
痰液	36	163	47	152	76.6
尿液	6	29	7	28	62.5
分泌物	28	48	30	46	87.1
其它	9	29	9	29	80.0

标本类型	涂片镜检法			培养法			符合率(%)
标本类型	阳性球菌	阴性杆菌	真菌	阳性球菌	阴性杆菌	真菌	阳性球菌	阴性杆菌	真菌
痰液	1	21	14	2	30	15	50.0	70.0	93.3
尿液	2	3	1	3	3	1	66.7	50.0	100.0
分泌物	3	24	1	4	25	1	75.0	88.5	100.0
其它	3	6	0	2	7	0	66.7	85.7	0.0

The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured

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