The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured

doi:10.3969/j.issn.1673-8640.2015.03.014

Abstract

Abstract: Objective

To investigate the clinical significance of microbial specimens being smeared and Gram stained for microscopy before they were cultured .

Methods

A total of 365 clinical specimens with different types of microorganisms were firstly smeared and stained for microscopy, then were inoculated for culture. Finally, the coincidence rate was analyzed.

Results

Among the 365 specimens, there were 211 sputum specimens, 40 urine specimens, 76 secretion specimens and 38 other-type specimens (mainly pus and pleural effusion specimens). Among them, there were 17 unqualified specimens. Of the 348 qualified specimens, 93 cases were cultured positive. The detection results of sputum, urine, secretion and other-type specimens were no statistical significance by the smear microscopy and the culture method. The coincidence rates were 76.6%, 62.5%, 87.1% and 80.0%, respectively (P>0.05).

Conclusions

The smear microscopy can not only check the quality of specimens, but also reduce the result errors of culture, so as to improve the positive rate of specimens by culture. Therefore, it is of great significance in the actual microbiological testing work.

Key words: Smear, Microscopy, Culture, Microbial specimen

CLC Number:

R446.5

ZHANG Guoying, XIA Xuehong. The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured[J]. Laboratory Medicine, 2015, 30(3): 258-261.

标本类型	菌种	例数
痰液
	肺炎克雷伯菌	19
	白念珠菌	15
	铜绿假单胞菌	4
	嗜麦芽窄食单胞菌	3
	溶血葡萄球菌	2
	其它	4
	合计	47
尿液
	屎肠球菌	3
	产气肠杆菌	1
	恶臭假单胞菌	1
	大肠埃希菌	1
	白念珠菌	1
	合计	7
分泌物
	大肠埃希菌	17
	肺炎克雷伯菌	3
	铜绿假单胞菌	2
	金黄色葡萄球菌	2
	白念珠菌	1
	肺炎链球菌	1
	其它	4
	合计	30
其它
	大肠埃希菌	4
	铜绿假单胞菌	2
	木糖葡萄球菌	1
	金黄色葡萄球菌	1
	液化沙雷菌	1
	合计	9

标本类型	菌种	例数
痰液
	肺炎克雷伯菌	19
	白念珠菌	15
	铜绿假单胞菌	4
	嗜麦芽窄食单胞菌	3
	溶血葡萄球菌	2
	其它	4
	合计	47
尿液
	屎肠球菌	3
	产气肠杆菌	1
	恶臭假单胞菌	1
	大肠埃希菌	1
	白念珠菌	1
	合计	7
分泌物
	大肠埃希菌	17
	肺炎克雷伯菌	3
	铜绿假单胞菌	2
	金黄色葡萄球菌	2
	白念珠菌	1
	肺炎链球菌	1
	其它	4
	合计	30
其它
	大肠埃希菌	4
	铜绿假单胞菌	2
	木糖葡萄球菌	1
	金黄色葡萄球菌	1
	液化沙雷菌	1
	合计	9

标本类型	涂片镜检法		培养法		符合率 (%)
标本类型	阳性	阴性	阳性	阴性	符合率 (%)
痰液	36	163	47	152	76.6
尿液	6	29	7	28	62.5
分泌物	28	48	30	46	87.1
其它	9	29	9	29	80.0

标本类型	涂片镜检法		培养法		符合率 (%)
标本类型	阳性	阴性	阳性	阴性	符合率 (%)
痰液	36	163	47	152	76.6
尿液	6	29	7	28	62.5
分泌物	28	48	30	46	87.1
其它	9	29	9	29	80.0

标本类型	涂片镜检法			培养法			符合率(%)
标本类型	阳性球菌	阴性杆菌	真菌	阳性球菌	阴性杆菌	真菌	阳性球菌	阴性杆菌	真菌
痰液	1	21	14	2	30	15	50.0	70.0	93.3
尿液	2	3	1	3	3	1	66.7	50.0	100.0
分泌物	3	24	1	4	25	1	75.0	88.5	100.0
其它	3	6	0	2	7	0	66.7	85.7	0.0

The clinical significance of smear and Gram staining for microscopy before microbial specimens being cultured

RichHTML

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

Figures/Tables 3

References 7

Related Articles 15

Recommended Articles

Metrics

[1]	蒋开龙,周波,杨志.送检细菌标本直接涂片染色镜检的意义[J].实用医技杂志,2011,18(5):505.
[2]	倪语星,尚红.临床微生物学与检验[M]. 4版. 北京:人民卫生出版社,2009:564-565.
[3]	骆永年,梁捷.细菌培养标本涂片镜检的意义[J].中国医药指南,2010, 8(20):70-71.
[4]	袁飞,万莉,谢帮才.358例标本涂片镜检与培养结果的相关性分析[J].中国实验诊断学,2008,12(6):771-773.
[5]	杨朵,辛续丽,马东媛,等.痰培养标本合格性评估标准的比较[J].检验医学,2012,27(9):773-775.
[6]	邓宁. 培养前标本涂片检查在临床辅助诊断中的应用评价[J].甘肃医药,2012, 31(3):179-181.
[7]	陈险峰,周庭银.痰标本涂片革兰染色镜检的临床意义[J].检验医学,2013, 28(6):499-502.

[1]	WU Qi, ZOU Yan, ZHANG Yanxia, CHEN Ligang. Diagnostic value of Xpert MTB/RIF combined with BD-960 rapid culture and Roche culture in BALF for bacteriological negative pulmonary tuberculosis [J]. Laboratory Medicine, 2025, 40(8): 793-797.
[2]	LIU Shanfeng, GAO Yun, WANG Limin, WANG Ping. Accuracy analysis of urine protein and urine cast of hospitalized liver cirrhosis patients [J]. Laboratory Medicine, 2025, 40(3): 287-293.
[3]	LUO Yuanmei, LIU Chang, CHEN Lilong, LI Zhenzhang, YUE Yubiao, LI Yang, XU Lingqing. Acute promyelocytic leukemia diagnosis assisted by YOLOV8 deep learning model [J]. Laboratory Medicine, 2025, 40(10): 998-1003.
[4]	YANG Huilin, CHEN Juan, YAN Jinjin, OU Jiawen, WEN Mingming, ZHOU Lina. Analysis of 4 blood culture bottles' adsorption capacity to 4 commonly used antibiotics [J]. Laboratory Medicine, 2024, 39(6): 578-582.
[5]	Laboratory Medicine Branch of Chinese Association of Geriatric Health Medicine Research, the Laboratory Medicine Committee of Shanghai Scientific Association of Better Birth and Better Upbringing （Shanghai Maternal and Child Health Association）. Expert consensus on establishment and validation of review rules for automatic vaginal secretion analysis system [J]. Laboratory Medicine, 2024, 39(5): 415-422.
[6]	LI Anqi, ZHANG Shuya, HE Shanna, CHEN Qiaoqiao, LÜ Qi, LÜ Wanfei, LI Chunxian, WANG Weihua, ZHENG Lin. Rapid bacterial identification and drug susceptibility test of blood culture-positive samples [J]. Laboratory Medicine, 2024, 39(3): 243-248.
[7]	XU Chunhui, ZHOU Xinyue, YI Huiming, ZHANG Lining, CHEN Shulian, ZHU Guoqing, FENG Sizhou. Diagnostic roles of mNGS and Mucorales PCR in pulmonary mucormycosis in patients with blood diseases [J]. Laboratory Medicine, 2024, 39(12): 1145-1149.
[8]	MA Yan, ZHOU Chunmei, HUANG Shenglei, WANG Beili, PAN Baishen, GUO Wei. Application evaluation of 24 h satellite blood culture to improve pathogenic microorganism culture process in emergency [J]. Laboratory Medicine, 2024, 39(10): 993-998.
[9]	DAI Fangfang, LU Xinxin, YU Yanhua, CHEN Ming, SUN Guizhen. Analysis of Mycobacterium infection in HIV/AIDS patients [J]. Laboratory Medicine, 2023, 38(12): 1173-1176.
[10]	LU Tingyan, GU Danfeng, WANG Yahong, GE Yafang, YANG Haiou. Evaluation of vaginal secretion routine determination modes and review criteria analysis [J]. Laboratory Medicine, 2023, 38(11): 1091-1097.
[11]	LI Daming, LÜ Xing, JIANG Chuanhao, HU Min. Clinical application of gene chip technology for determining respiratory pathogens rapialy in pulmonary infection patients after renal transplantation [J]. Laboratory Medicine, 2022, 37(9): 864-867.
[12]	PAN Fen, PAN Yuxuan, SHI Yingying, YU Fangyuan, ZHANG Hong. UroQuick rapid urine culture technique for urinary tract infection in children [J]. Laboratory Medicine, 2022, 37(7): 652-656.
[13]	CHEN Hanlu, WU Shenghai. Influence factors of blood culture negative results and progress of pathogen detection in bloodstream infection [J]. Laboratory Medicine, 2022, 37(7): 688-694.
[14]	CHU Minjun, LI Xiaojuan, HUANG Hailin, LI Jue, MA Mingbiao, DU Tingyi, WANG Haiping. Influence of delayed entry on fastidious bacterium culture in 2 blood culture systems [J]. Laboratory Medicine, 2022, 37(3): 209-212.
[15]	WANG Juan, LIAO Huanjin, LI Yanning, GE Yiqin, LI Jia. RSV infection of air-liquid interface cultured human bronchial epithelial cell and its effect on HMGB1 and pMLKL expressions [J]. Laboratory Medicine, 2022, 37(2): 177-182.