Application of the combination detection of serum AFP, GP73 and GPC3 on the diagnosis of primary hepatic carcinoma

doi:10.3969/j.issn.1673-8640.2014.09.003

Abstract

Abstract:

Objective To investigate the application significance of the combination detection of alpha-fetoprotein (AFP), Golgi protein 73(GP73) and glypican 3(GPC3) on the diagnosis of primary hepatic carcinoma (PHC). Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of GP73 and GPC3 in 148 patients with PHC, 73 patients with liver cirrhosis (LC), 57 patients with chronic hepatitis B (CHB) and 85 healthy subjects(healthy control group). Serum AFP was detected by chemiluminescence immunoassay. The levels of GP73 and GPC3 were compared with that of AFP. Results Serum levels of GP73, GPC3 and AFP in PHC group were 346.9±208.7, 143.1±66.9 and (690.3±402.5) μg/L, which were significant statistically compared with those inLC, CHB and healthy control groups (P<0.01). In the combination detection, the sensitivity was 95.9%, and the accuracy was 89.2%. Conclusions The combination detection of serum AFP, GP73 and GPC3 could cover the shortage of individual detections, increase the diagnosis rate of PHC and differentiate PHC with clinical significance.

Key words: Alpha-fetoprotein, Golgi protein 73, Glypican 3, Combination detection, Primary hepatic carcinoma

CLC Number:

R446.62

FAN Gongren, WANG Weifang, HAN Juqiang, JIANG Shengying, CAO Jianbiao. Application of the combination detection of serum AFP, GP73 and GPC3 on the diagnosis of primary hepatic carcinoma[J]. , 2014, 29(9): 884-888.

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