Abstract: Objective Through detecting methicillin-resistant Staphylococcus aureus (MRSA)with mecA gene related hyper-variable region (HVR) genotyping to understand the nosocomial infection outbreak of MRSA. Methods By polymerase chain reaction (PCR), the HVR of MRSA mecA gene from 38 isolates of MRSA was amplified. According to size differences, the amplified fragments were genotyped. Results According to the PCR fragment size, 38 isolates of MRSA were classified into A, B, C and D genotypes respectively, in which C genotype was the most common type (23 isolates, 60.53% ), followed by A genotype (7 isolates, 18.42%), B genotype (3 isolates, 7.89%), D genotype (2 isolates, 5.26%) and no genotype (3 isolates, 7.89%). C genotype distributed in various departments, mainly in cadre department. MRSA showed serious multi-drug resistance. Therefore, the first choice of antibiotic therapy was vancomycin,teicoplanin/dalfopristin and tigecycline. Conclusions There was a spread of nosocomial MRSA cloning in hospital, and it should be paid attention to strengthen the detection and control of nosocomial infection outbreak.

Key words: Methicillin-resistant Staphylococcus aureus, MecA gene related hyper-variable region genotyping, Genotype, Nosocomial infection, Outbreak