Abstract: Objective　To evaluate the application significance of pregnancy test strip for the simultaneous detection of serum and urine human chorionic gonadotrophin（HCG）in the diagnosis of ectopic pregnancy（EP）in woman acute abdomen.  Methods　A total of 362 cases diagnosed as EP in patients with woman acute abdomen and 308 non-pregnant patients with woman acute abdomen were enrolled. Their serum and urine samples were collected. The samples were detected for serum and urine HCG by pregnancy test strip. The performance results of the single detection of serum or urine HCG and the simultaneous detection of serum and urine HCG were compared and analyzed.  Results　In woman acute abdomen，the sensitivity of the simultaneous detection of serum and urine HCG was 100%，which was obviously higher than that of the single detection of urine HCG（73.2%，P＜0.01），and there was no statistical significance with that of the single detection of serum HCG（P＞0.05）. When the results for serum and urine HCG were all positive，the positive predictive value for the diagnosis of EP was 100%. When the results for serum and urine HCG were all negative，the negative predictive value for the diagnosis of EP was 100%.  Conclusions　In the differential diagnosis of woman acute abdomen，the simultaneous detection of serum and urine HCG by pregnancy test strip could improve the detection rate of EP，and could make up for the insufficiency of single detection of serum or urine HCG effectively. It has convenience and reasonable price，and is suitable for the hospitals without the quantitation detection of serum HCG.

Key words: Pregnancy test strip, Human chorionic gonadotrophin, Blood, Urine, Woman acute abdomen