关键词: 核酸适配体荧光探针, 结核分枝杆菌, 液基夹层杯法, 结核病

Abstract:

Objective To compare the clinical application values of nucleic acid aptamer fluorescence probe method and liquid-based sandwich cup method in the determination of Mycobacterium tuberculosis（MTB）. Methods Sputum samples from 175 patients with pulmonary tuberculosis were collected and determined by liquid culture method，solid culture method，acid fast staining，liquid-based sandwich cup method and nucleic acid aptamer fluorescence probe method. Liquid culture method was used as the reference standard. The consistency of the results of nucleic acid aptamer fluorescence probe method，liquid-based sandwich cup method and liquid culture method was evaluated. Results Compared with the results of liquid culture method，the sensitivity of liquid-based sandwich cup method was 78.08%，and the specificity was 98.04%. The sensitivity of nucleic acid aptamer fluorescence probe method was 54.79%，and the specificity was 50.98%. Conclusions Liquid-based sandwich cup method is consistent with liquid culture method，and it has good clinical diagnostic value. Nucleic acid aptamer fluorescence probe method needs to be further optimized.

Key words: Nucleic acid aptamer fluorescence probe, Mycobacterium tuberculosis, Liquid-based sandwich cup method, Tuberculosis