关键词: 粒细胞巨噬细胞集落刺激因子, 可溶性髓系细胞触发受体-1, 结核感染T细胞斑点试验, 肺结核

Abstract:

Objective To investigate the roles of serum granulocyte macrophage colony stimulating factor（GM-CSF）,soluble triggering receptor expressed on myeloid cell-1（sTREM-1） combined with T-cell spot test for tuberculosis infection（T-SPOT.TB） in the differential diagnosis of pulmonary tuberculosis. Methods A total of 94 patients with pulmonary tuberculosis were enrolled as observation group,and 94 patients with other lung diseases were enrolled as control group. Serum GM-CSF and sTREM-1 levels were determined,and T-SPOT.TB was performed simultaneously. Logistic regression analysis was used to establish the equation of GM-CSF,sTREM-1 and T-SPOT.TB combined determination for the diagnosis of pulmonary tuberculosis. The diagnostic efficiency of GM-CSF,sTREM-1 and T-SPOT.TB in the diagnosis of pulmonary tuberculosis was evaluated by receiver operating characteristic（ROC） curve. Results The levels of GM-CSF and sTREM-1 in observation group were higher than those in control group（P=0.000）. Taking sputum smear or culture test results as standard,the areas under curves（AUC） of GM-CSF,sTREM-1,T-SPOT.TB single determinations and combined determination for the diagnosis of pulmonary tuberculosis were 0.674,0.687,0.758 and 0.832,respectively. The combined determination of the 3 indicators was more effective than single determinations in diagnosing pulmonary tuberculosis（P<0.05）. Conclusions GM-CSF,sTREM-1 combined determination with T-SPOT.TB can effectively improve the diagnostic efficiency of pulmonary tuberculosis.

Key words: Granulocyte macrophage colony stimulating factor, Soluble triggering receptor expressed on myeloid cell-1, T-cell spot test for tuberculosis infection, Pulmonary tuberculosis