干热法灭活对新型冠状病毒肺炎相关炎症指标检测的影响

doi:10.3969/j.issn.1673-8640.2020.09.016

摘要/Abstract

摘要：

目的探讨血样本经56 ℃ 30 min干热灭活后,对新型冠状病毒肺炎（COVID-19）诊疗相关炎症因子检测结果的影响程度。方法收集进行过C反应蛋白（CRP）、降钙素原（PCT）及白细胞介素6（IL-6）检测,且结果尽量覆盖线性范围的临床剩余血样本332例。所有样本于收集当天进行56 ℃ 30 min干热灭活,比较灭活前、后检测结果的差异。结果 CRP灭活前、后检测结果差异无统计学意义（P=0.167）,IL-6、PCT灭活前、后检测结果差异有统计学意义（P值分别为0.002和<0.001）。有98例（79.03%）样本灭活后PCT水平下降。灭活前、后3个炎症指标结果均有较好的一致性,PCT、CRP及IL-6分别有96.77%、97.11%及97.11%的散点位于差值的95%可信区间,相关系数（r值）分别为0.992、0.999和0.996。结论 56 ℃ 30 min干热灭活对CRP的检测结果无影响,对PCT、IL-6的检测结果有影响。建议实验室关注COVID-19患者样本传染性风险的前处理流程。

关键词: 新型冠状病毒肺炎, 干热法灭活, 炎症指标, C反应蛋白, 降钙素原, 白细胞介素6

Abstract:

Objective To investigate the influence of dry heat inactive treatment（56 ℃ 30 min） for the determination of inflammatory indicators related with corona virus disease 2019（COVID-19）diagnosis and treatment. Methods Totally,332 clinical residual blood samples which C-reactive protein（CRP）,procalcitonin（PCT） and interleukin 6（IL-6） determinations were carried out were collected. All the samples were inactivated by dry heat inactive treatment at 56℃ for 30 min on the day of collection,and the difference of results was compared. Results There was no statistical significance in CRP determination results before and after inactivation（P=0.167）,and there was statistical significance in IL-6 and PCT determination results before and after inactivation（P=0.002,P<0.001）,of which the PCT determination results of 98（79.03%） samples decreased after inactivation. The determination results of the 3 inflammatory indicators before and after inactivation had good consistency,and PCT,CRP and IL-6 had 96.77%,97.11% and 97.11% scatter points in 95% confidence intervals,respectively. The correlation coefficients （r） were 0.992,0.999 and 0.996,respectively. Conclusions Dry heat inactive treatment at 56℃ for 30 min has no effect on CRP determination results,but has effect on PCT and IL-6 determination results. It should pay attention to the pretreatment process of dry heat inactive treatment,in order to reduce the infectious risk of COVID-19 patients' samples.

Key words: Corona virus disease 2019, Dry heat inactive treatment, Inflammatory indicator, C-reactive protein, Procalcitonin, Interleukin 6

中图分类号:

R446.1

赵明海, 李欣, 魏东, 罗敏, 张雅洁, 杨懿祺, 郑磊. 干热法灭活对新型冠状病毒肺炎相关炎症指标检测的影响[J]. 检验医学, 2020, 35(9): 924-927.

ZHAO Minghai, LI Xin, WEI Dong, LUO Min, ZHANG Yajie, YANG Yiqi, ZHENG Lei. Influence of dry heat inactive treatment for the determination of inflammatory indicators related with corona virus disease 2019[J]. Laboratory Medicine, 2020, 35(9): 924-927.

图/表 3

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项目	例数	PCT/（ng/mL）	CRP/（pg/mL）	IL-6/（pg/mL）
灭活前	332	0.701（0.064,10.530）	24.735（4.960,86.765）	33.070（15.317,130.725）
灭活后	332	0.644（0.066,10.050）	23.995（4.922,84.778）	32.320（16.352,127.605）
统计值		-7.352	-1.381	-3.150
P值		<0.001	0.167	0.002

项目	例数	PCT/（ng/mL）	CRP/（pg/mL）	IL-6/（pg/mL）
灭活前	332	0.701（0.064,10.530）	24.735（4.960,86.765）	33.070（15.317,130.725）
灭活后	332	0.644（0.066,10.050）	23.995（4.922,84.778）	32.320（16.352,127.605）
统计值		-7.352	-1.381	-3.150
P值		<0.001	0.167	0.002