检验医学 ›› 2018, Vol. 33 ›› Issue (5): 384-387.DOI: 10.3969/j.issn.1673-8640.2018.05.002

• 临床应用研究·论著 • 上一篇    下一篇

解脲棒状杆菌与尿液中磷酸铵镁结晶形成的相关性

李采青, 曹静, 詹明华, 韩旭颖, 李小凤, 刘金禄   

  1. 河北北方学院附属第一医院微生物科,河北 张家口 075000
  • 收稿日期:2017-05-15 出版日期:2018-05-20 发布日期:2018-05-30
  • 作者简介:null

    作者简介:李采青,女,1969年生,学士,副主任技师,主要从事病原微生物学研究。

Relationship between Corynebacterium urealyticum and the formation of ammonium magnesium phosphate crystals in urine

LI Caiqing, CAO Jing, ZHAN Minghua, HAN Xuying, LI Xiaofeng, LIU Jinlu   

  1. Laboratory of Microbiology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China
  • Received:2017-05-15 Online:2018-05-20 Published:2018-05-30

摘要:

目的 了解解脲棒状杆菌在磷酸铵镁结晶阳性尿液中的地位和促进磷酸铵镁结晶形成的作用,为临床积极抗感染治疗,防止感染性结石形成提供依据。方法 对2015年1月—2016年12月来河北北方学院附属第一医院就诊患者的磷酸铵镁结晶阳性尿液进行细菌培养,分析其细菌构成。将解脲棒状杆菌置于新鲜无细菌、无其他有形成分尿液中进行培养,24和48 h后分别用超高倍显微镜观察磷酸铵镁结晶的形成情况。结果 共有46例尿液样本检出磷酸铵镁结晶,25例细菌培养阳性(男17例、女8例),其中12例培养出2种病原菌。解脲棒状杆菌8株(阳性率为32.0%,其中7株来自男性)、肠球菌8株、奇异变形杆菌6株、大肠埃希菌5株、肺炎克雷伯菌3株、白念珠菌3株、铜绿假单胞菌2株。在8株解脲棒状杆菌中有4株与其他病原菌混合生长。在无磷酸铵镁结晶的解脲棒状杆菌培养液中观察到磷酸铵镁结晶形成,以未成形羽毛状结晶最多,立方体、方柱状、洋信封状结晶均可见。结论 解脲棒状杆菌是特定人群泌尿系统感染和感染性结石形成的重要病原菌之一。

关键词: 解脲棒状杆菌, 磷酸铵镁结晶, 泌尿系统感染

Abstract:

Objective To study the role of Corynebacterium urealyticum in the formation of ammonium magnesium phosphate crystals in urine,and to provide a reference for the prevention of urinary calculi. Methods Urine samples with ammonium magnesium phosphate crystals were collected from the First Affiliated Hospital of Hebei North University from January 2015 to December 2016, Corynebacterium urealyticum was cultured in the fresh urine without bacteria and other urine formed elements. After culturing for 24 and 48 h,the formation of ammonium magnesium phosphate crystals was observed by ultrahigh sensitive microscope. Results Bacteria in urine samples of 17 males and 8 females were isolated in 46 cases of urine samples with ammonium magnesium phosphate crystals, and 12 cases had 2 pathogens. There were 8 isolates of Corynebacterium urealyticum,the positive rate was 32.0%(8/25),and there were 7 isolates from males. There were 8 isolates of Enterococcus,6 isolates of Proteus mirabilis,5 isolates of Escherichia coli,3 isolates of Klebsiella pneumoniae,3 isolates of Candida albicans and 2 isolates of Pseudomonas aeruginosa. There were 4 of 8 isolates of Corynebacterium urealyticum mixed with other pathogens. Ammonium magnesium phosphate crystals were observed from urine culture fluid. Most of the crystals formed were unformed feather crystals, followed by cubic, square cylinder and envelope-like crystals. Conclusions Corynebacterium urealyticum is one of the main pathogens of urinary tract infection and urinary calculus formation for specific population.

Key words: Corynebacterium urealyticum, Ammonium magnesium phosphate crystal, Urinary tract infection

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