α-硫辛酸对肥胖模型大鼠血清脂肪因子的影响

doi:10.3969/j.issn.1673-8640.2015.11.020

检验医学 ›› 2015, Vol. 30 ›› Issue (11): 1147-1149.DOI: 10.3969/j.issn.1673-8640.2015.11.020

α-硫辛酸对肥胖模型大鼠血清脂肪因子的影响

邵云¹, 秦小霞², 魏从真², 张秋芬³, 邵明³, 闫国超²

1.河北医科大学第一医院超声科,河北石家庄 050031
2.河北医科大学第一医院检验科,河北石家庄 050031
3.石家庄市桥西区振头社区卫生服务中心,河北石家庄 050031

收稿日期:2015-02-21 出版日期:2015-11-30 发布日期:2015-12-03
作者简介:null
作者简介:邵云,女,1977年生,学士,副主任技师,主要从事临床超声诊断工作。

通讯作者:闫国超,联系电话:0311-85917323。

Influence of alpha-lipoic acid on serum adipocytokines in a rat model of dietary-induced obesity

SHAO Yun¹, QIN Xiaoxia², WEI Congzhen², ZHANG Qiufen³, SHAO Ming³, YAN Guochao²

1 .Department of Ultrasonography, the First Hospital of Hebei Medical University,Hebei Shijiazhuang 050031, China
2. Department of Clinical Laboratory,the First Hospital of Hebei Medical University, Hebei Shijiazhuang 050031,China
3. Zhentou Community Health Center of Qiaoxi District, Hebei Shijiazhuang 050031,China

Received:2015-02-21 Online:2015-11-30 Published:2015-12-03

摘要/Abstract

摘要： 目的

探讨α-硫辛酸(ALA)对饮食诱导肥胖大鼠脂肪因子的影响。

方法

选取清洁级8周龄雄性Wistar大鼠50只,随机选其中10只,以普通饲料喂养作为对照组(NC);高脂饮食成功诱导肥胖大鼠模型32只,随机分为高脂饲料(HFD)组(16只)和HFD+ALA组[16只,腹腔注射ALA(30 mg/kg)2周]。测定各组大鼠血清氧化应激、脂肪因子等指标并进行统计学分析。

结果

HFD组血清丙二醛(MDA)、瘦素、肿瘤坏死因子-α(TNF-α)分别为(8.8±0.7)μmol/L、(9.8±0.8)ng/mL、(11.6±1.2)ng/L,明显高于HFD+ALA组[(6.1±0.6)μmol/L、(7.6±0.7)ng/mL、(9.7±2.3)ng/L]和NC组[(7.9±0.4)μmol/L、(4.6±0.6)ng/mL、(8.8±3.5)ng/L](P均<0.05);而超氧化物歧化酶(SOD)[(73.9±5.1)U/mL]、总抗氧化能力(T-AOC)[(18.0±3.6)U/mL]、脂联素[(8.7±1.5)mg/L]明显低于HFD+ALA组[(80.9±1.8)U/mL、(25.8±5.4)U/mL、(12.6±2.1)mg/L]和NC组 [(81.0±3.2)U/mL、(20.9±2.3)U/mL、(14.0±3.4)mg/L] (P均<0.05)。与NC组比较,HFD+ALA组血清T-AOC、瘦素明显升高(P均<0.05),MDA明显下降(P<0.05),SOD、脂联素、TNF-α差异无统计学意义(P均>0.05)。相关性分析显示血清MDA与瘦素、TNF-α呈明显正相关[相关系数(r)值分别为0.716、0.502,P均<0.01];T-AOC与脂联素呈负相关(r=-0.485,P<0.05)。

结论

ALA可改善肥胖大鼠的氧化应激状态并影响脂肪因子的分泌。

关键词: α-硫辛酸, 脂肪因子, 肥胖, 动物模型, 氧化应激

Abstract: Objective

To investigate the influence of alpha-lipoic acid (ALA)on serum adipocytokines in a rat model of dietary-induced obesity.

Methods

A total of 50 Wistar rats were randomly classified into 3 groups, a negative control (NC)group fed with normal chow (10 cases), high fat diet(HFD)group fed with a high-fat diet (16 cases) and HFD+ALA group fed with a high-fat diet with an intervention of ALA(30mg/kg, 2 weeks,16 cases).The concentrations of serum oxidative stress biomarkers and adipocytokines were determined, and the results were analyzed statistically.

Results

The levels of serum malondialdehyde(MDA), leptin and tumor necrosis factor-alpha (TNF-α) in HFD group were (8.8±0.7)μmol/L , (9.8±0.8)ng/mL and (11.6±1.2)ng/L, which were higher than those in HFD+ALA group [(6.1±0.6)μmol/L , (7.6 ±0.7)ng/mL and (9.7±2.3)ng/L] and NC group [(7.9±0.4)μmol/L, (4.6±0.6)ng/mL and (8.8±3.5)ng/L] (P<0.05). The levels of superoxide dismutase(SOD) [(73.9±5.1)U/mL], total antioxidant capacity(T-AOC) [(18.0±3.6)U/mL] and adiponectin [(8.7±1.5) mg/L] were lower than those in HFD+ALA group [(80.9±1.8)U/mL, (25.8±5.4)U/mL and(12.6±2.1)mg/L] and NC group [(81.0±3.2)U/mL,(20.9±2.3)U/mL and (14.0±3.4)mg/L](P<0.05). Compared with NC group, the levels of serum T-AOC and leptin were much higher in HFD+ALA group (P<0.05), MDA was much lower(P<0.05), and no statistical significance between the 2 groups was observed for SOD, adiponectin and TNF-α (P>0.05). Moreover, correlation analysis showed that serum MDA , leptin and TNF- α showed a significantly positive correlation[correlation coefficients (r)=0.716 and 0.502, P<0.01]. A negative correlation of T-AOC with adiponectin (r=-0.485,P< 0.05).

Conclusions

ALA can improve the oxidative stress state and regulate adipocytokines in a rat model of dietary-induced obesity.

Key words: Alpha-lipoic acid, Adipocytokine, Obesity, Animal model, Oxidative stress

中图分类号:

R446.1

邵云, 秦小霞, 魏从真, 张秋芬, 邵明, 闫国超. α-硫辛酸对肥胖模型大鼠血清脂肪因子的影响[J]. 检验医学, 2015, 30(11): 1147-1149.

SHAO Yun, QIN Xiaoxia, WEI Congzhen, ZHANG Qiufen, SHAO Ming, YAN Guochao. Influence of alpha-lipoic acid on serum adipocytokines in a rat model of dietary-induced obesity[J]. Laboratory Medicine, 2015, 30(11): 1147-1149.

图/表 1

参考文献 10

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组别	例数	T-AOC (U/mL)	MDA (μmol/L)	SOD (U/mL)	瘦素 (ng/mL)	脂联素 (mg/L)	TNF-α (ng/L)
NC组	10	20.9±2.3	7.9±0.4	81.0±3.2	4.6±0.6	14.0±3.4	8.8±3.5
HFD组	16	18.0±3.6^*	8.8±0.7^*	73.9±5.1^*	9.8±0.8^*	8.7±1.5^*	11.6±1.2^*
HFD+ALA组	16	25.8±5.4^*#	6.1±0.6^*#	80.9±1.8^#	7.6±0.7^*#	12.6±2.1^#	9.7±2.3^#

组别	例数	T-AOC (U/mL)	MDA (μmol/L)	SOD (U/mL)	瘦素 (ng/mL)	脂联素 (mg/L)	TNF-α (ng/L)
NC组	10	20.9±2.3	7.9±0.4	81.0±3.2	4.6±0.6	14.0±3.4	8.8±3.5
HFD组	16	18.0±3.6^*	8.8±0.7^*	73.9±5.1^*	9.8±0.8^*	8.7±1.5^*	11.6±1.2^*
HFD+ALA组	16	25.8±5.4^*#	6.1±0.6^*#	80.9±1.8^#	7.6±0.7^*#	12.6±2.1^#	9.7±2.3^#

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α-硫辛酸对肥胖模型大鼠血清脂肪因子的影响

Influence of alpha-lipoic acid on serum adipocytokines in a rat model of dietary-induced obesity

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