三例输入性卵形疟的实验室诊断

doi:10.3969/j.issn.1673-8640.2014.08.016

检验医学 ›› 2014, Vol. 29 ›› Issue (8): 851-855.DOI: 10.3969/j.issn.1673-8640.2014.08.016

三例输入性卵形疟的实验室诊断

田斌, 段绩辉, 徐明忠, 张兵, 廖瑜, 申晓君, 文岚

长沙市疾病预防控制中心,湖南长沙 410001

收稿日期:2013-07-17 出版日期:2014-08-30 发布日期:2014-09-05
通讯作者: 通讯作者:段绩辉,联系电话:0731-84305786。
作者简介:田斌,男,1982年生,主管技师,主要从事病原微生物学及分子生物学检测。

Laboratory diagnosis on three cases of imported plasmodium ovale malaria infection

TIAN Bin, DUAN Jihui, XU Mingzhong, ZHANG Bing, LIAO Yu, SHEN Xiaojun, WEN Lan

Changsha Center for Disease Control and Prevention, Hunan Changsha 410001, China

Received:2013-07-17 Online:2014-08-30 Published:2014-09-05

摘要/Abstract

摘要： 目的分析3例输入性疟疾的实验室诊断,减少卵形疟的误诊与漏诊。方法采用形态学检查、巢氏聚合酶链反应(PCR)及序列分析等方法确诊3例输入性疟疾病例。结果 3例患者形态学检查结果分别是未检出疟原虫(实验室初次检查)、检出卵形与恶性疟原虫和检出卵形疟原虫;巢氏PCR检测结果依次是卵形疟原虫感染、卵形与恶性疟原虫混合感染和卵形与间日疟原虫混合感染。结论需加强疟疾消除地区疟原虫形态学检查技术的培训和推广。应用分子生物学检查技术可减少输入性卵形疟的误诊与漏诊。

关键词: 卵形疟原虫, 巢氏PCR, 序列分析, 混合感染, 实验室诊断

Abstract: Objective To analyze 3 cases of imported malaria infection, in order to reduce the misdiagnosis and missed diagnosis of ovale malaria infection. Methods By morphological test, nested polymerase chain reaction (PCR) and sequence analysis were used to confirm the 3 cases of imported malaria infection. Results The morphological test results showed no plasmodium (laboratory initial inspection), plasmodium ovale mixed infection with plasmodium falciparum and plasmodium ovale. However, plasmodium ovale, plasmodium ovale mixed infection with plasmodium falciparum and plasmodium ovale mixed infection with plasmodium vivax were detected by nested PCR by follow. Conclusions The morphological technique training and the application of molecular biology techniques should be strengthen for imported plasmodium ovale malaria infection, and can reduce the misdiagnosis and missed diagnosis of imported plasmodium ovale malaria infection.

Key words: Plasmodium ovale, Nested polymerase chain reaction, Sequence analysis, Mixed infection, Laboratory diagnosis

中图分类号:

R382.3

田斌, 段绩辉, 徐明忠, 张兵, 廖瑜, 申晓君, 文岚. 三例输入性卵形疟的实验室诊断[J]. 检验医学, 2014, 29(8): 851-855.

TIAN Bin, DUAN Jihui, XU Mingzhong, ZHANG Bing, LIAO Yu, SHEN Xiaojun, WEN Lan. Laboratory diagnosis on three cases of imported plasmodium ovale malaria infection[J]. , 2014, 29(8): 851-855.

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三例输入性卵形疟的实验室诊断

Laboratory diagnosis on three cases of imported plasmodium ovale malaria infection

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