关键词: 组织蛋白酶D, 浆膜腔积液, 肿瘤, 受试者工作特征曲线

Abstract:

Objective　To study the relationship between tumor metastasis and cathepsin D(CD) in serous cavity effusion. Methods　A total of 77 patients with serous cavity effusion were enrolled in the study. According to the clinical data and pathological diagnosis,the 77 patients were classified into 2 groups,non-tumor group(22 cases) and tumor group (55 cases, including 30 cases of tumor cell positive group and 25 cases of tumor cell negative group). The concentrations of CD and carcinoembryonic antigen (CEA) in serous cavity effusion were determined by enzyme-linked immunosorbent assay (ELISA) and chemiluminescence, respectively. The concentrations of CD and CEA from different groups were compared, and the receiver operating characteristic(ROC) curve was used to evaluate the diagnosis significance of CD determination, CEA determination and the combined CD and CEA determination. Results　The concentrations of CD and CEA in the tumor group were significantly higher than those in the non-tumor group (P<0.05). The concentration of CD was positively correlated with that of CEA(Kendall correlation coefficient 0.323, Spearman correlation coefficient 0.396, P<0.001). The areas under the ROC curve of CD,CEA and CEA combined with CD for discriminating tumor were 0.763,0.723 and 0.812, respectively. The concentration of CD in the tumor cell positive group was significantly higher than that in the tumor cell negative group(P<0.05),whereas the concentrations of CEA in the 2 groups were not significantly different (P=0.051). The areas under the ROC curve of CD,CEA and CEA combined with CD for discriminating tumor metastasis were 0.677, 0.654 and 0.767, respectively. Conclusions　The concentration of CD in serous cavity effusion is related to tumor serous cavity metastasis. CD in serous cavity effusion may be a candidate indicator for discriminating tumor and tumor serous cavity metastasis. The combined CD and CEA determination is recommended.

Key words: Cathepsin D, Serous cavity effusion, Tumor, Receiver operating characteristic curve